Mapping of Qtls related with wood quality and developmental characteristics in hybrids (Eucalyptus grandis X Eucalyptus urophylla)

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Universidade Federal de Viçosa

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The present work aimed to characterize and identify QTLs for wood quality and growth traitsin E. grandis x E. urophylla hybrids. For this purpose a RAPD linkage map was developed for the hybrids(LOD=3 and r=0.40) containing 52 markers and 12 linkage groups. Traits related to wood quality and growthwere evaluated in the QTL analyses. QTL analyses were performed using chi-square tests, single-marker, intervalmapping and composite interval mapping analyses. All approaches led to the identification of similar QTLsassociated with wood density, cellulose pulp yield and percentage of extractives, which were detected andconfirmed by both the interval mapping and composite interval mapping methodologies. Some QTLs regionswere confirmed only by the composite interval mapping methodology: percentage of soluble lignin, percentageof insoluble lignin, CBH and total height. Overlapping QTLs regions were detected, and these, can be theresult of major genes involved in the regulation and control of the growth traits by epistatic interactions.In order to evaluate the effect of early selection using RAPD molecular data, molecular markers adjacentto QTLs were used genotype selection. The analysis of selection differential values suggests that for all thetraits the phenotypic selection at seven years should generate larger genetic gains than early selection assistedby molecular markers and the combination of the strategies should elevate the selection efficiency.

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