Development of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars.

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dc.contributorANA LUISA SOUSA AZEVEDO, CNPGL; FLÁVIA RANGEL DE SOUZA; ROSIANA ANGÉLICA CAMPOS; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; JUAREZ CAMPOLINA MACHADO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; FRANCISCO JOSE DA SILVA LEDO, CNPGL; MARCIO RESENDE, University of Florida.
dc.creatorAZEVEDO, A. L. S.
dc.creatorSOUZA, F. R. de
dc.creatorCAMPOS, R. A.
dc.creatorREIS, D. R. de L.
dc.creatorMACHADO, J. C.
dc.creatorMACHADO, M. A.
dc.creatorLEDO, F. J. da S.
dc.creatorRESENDE, M.
dc.date2022-11-11T18:16:24Z
dc.date2022-11-11T18:16:24Z
dc.date2022-11-11
dc.date2022
dc.date.accessioned2026-07-07T03:48:55Z
dc.descriptionNapier grass is a perennial tropical forage that is used in beef and dairy production systems. Despite its significance in animal nutrition, molecular information available, such as microsatellite or simple sequence repeat (SSR) or single nucleotide polymorphism (SNP) markers, is limited. Using an assembled transcriptome, 50 novel SSR markers were developed, of which 21 were found to be polymorphic. These polymorphic markers were tested for DNA fingerprinting of Embrapa cultivars, five of which revealed distinct allele patterns for cultivar identification. SSR markers 05, 17, and 44 identified a unique pattern in the BRS Kurumi cultivar. The BRS Capiaçu cultivar was identified using SSR markers 17, 43, and 44. The Pioneiro cultivar exhibited a rare fragment amplification pattern using SSR marker 46, while SSR marker 44 revealed a distinct allele in the BRS Canará cultivar. SSR marker panels could be utilized as DNA fingerprinting tools to assist in cultivar identification.
dc.descriptionNota científica.
dc.identifierCrop Breeding and Applied Biotechnology, v. 22, n. 4, e42522244, 2022.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1148220
dc.identifier.urihttp://hdl.handle.net/123456789/443806
dc.languageeng
dc.rightsopenAccess
dc.subjectBreeding program
dc.subjectSSR
dc.subjectElephant grass
dc.subjectCapim Elefante
dc.subjectGrama
dc.titleDevelopment of microsatellite panels for molecular fingerprinting of Napier grass (Cenchrus purpureus) cultivars.
dc.typeArtigo de periódico

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