Rapid CRISPR/Cas9 editing of genotype IX African swine fever virus circulating in eastern and central Africa

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dc.creatorAbkallo, Hussein M.
dc.creatorSvitek, Nicholas
dc.creatorOduor, Bernard
dc.creatorAwino, Elias
dc.creatorHenson, Sonal P.
dc.creatorOyola, Samuel O.
dc.creatorMwalimu, Stephen
dc.creatorAssad-Garcia, N.
dc.creatorFuchs, W.
dc.creatorVashee, S.
dc.creatorSteinaa, Lucilla
dc.date2021-08-30
dc.date2021-08-30T13:18:57Z
dc.date2021-08-30T13:18:57Z
dc.date.accessioned2026-06-27T16:54:40Z
dc.descriptionAfrican swine fever virus (ASFV) is the etiological agent of a contagious and fatal disease of domestic pigs that has significant economic consequences for the global swine industry. Due to the lack of effective treatment and vaccines against African swine fever, there is an urgent need to leverage cutting-edge technologies and cost-effective approaches for generating and purifying recombinant virus to fast-track the development of live-attenuated ASFV vaccines. Here, we describe the use of the CRISPR/Cas9 gene editing and a cost-effective cloning system to produce recombinant ASFVs. Combining these approaches, we developed a recombinant virus lacking the non-essential gene A238L (5EL) in the highly virulent genotype IX ASFV (ASFV-Kenya-IX-1033) genome in less than 2 months as opposed to the standard homologous recombination with conventional purification techniques which takes up to 6 months on average. Our approach could therefore be a method of choice for less resourced laboratories in developing nations.
dc.identifierhttps://hdl.handle.net/10568/114800
dc.identifier.urihttp://hdl.handle.net/123456789/136828
dc.languageen
dc.publisherFrontiers Media
dc.rightsOpen Access
dc.sourceAbkallo, H.M., Svitek, N., Oduor, B., Awino, E., Henson, S.P., Oyola, S.O., Mwalimu, S., Assad-Garcia, N., Fuchs, W., Vashee, S. and Steinaa, L. 2021. Rapid CRISPR/Cas9 editing of genotype IX African swine fever virus circulating in eastern and central Africa. Frontiers in Genetics 12: 733674.
dc.subjectafrican swine fever virus
dc.subjectanimal diseases
dc.subjectgenomics
dc.subjectcrispr
dc.titleRapid CRISPR/Cas9 editing of genotype IX African swine fever virus circulating in eastern and central Africa
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)