Fungal diversity associated with coffee leaf rust (Hemileia vastatrix) pustules based on ITS1 amplicon sequencin

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dc.contributorCentro Agronómico Tropical de Investigación y Enseñanza (CATIE)
dc.creatorSolano, William
dc.creatorBerkelmann, Dirk
dc.creatorZuñiga-Umaña, Juan Miguel
dc.creatorChaverri, Priscila
dc.creatorGatica-Arias, Andrés
dc.date2026-03-23T16:31:06Z
dc.date2026-03-23T16:31:06Z
dc.date2026-03-23
dc.date.accessioned2026-06-09T02:00:07Z
dc.descriptionCoffee leaf rust (CLR), caused by Hemileia vastatrix, is one of the biggest economic challenges for coffee cultivation and leads to high economic losses each year. Co-occurring fungal microbial communities and their diversity in the presence of CLR are widely understudied but may harbor potential agents or indicators to reduce CLR infections. In this study, the fungal communities associated with CLR pustules in Coffea arabica L. plants across different regions of Costa Rica were analyzed. To this end, individual pustules were excised from infected leaf tissue and used as source material for DNA extraction and subsequent amplification and sequencing of the fungal taxonomic marker region ITS1. Effects of altitude and location on fungal community structure were also observed. High taxonomic variance within regions and a large proportion of unclassified taxa were detected as well as similar community structures across regions, possibly reflecting small effects of the analyzed regions on the identified taxa. However, altitude was a significant factor on the detected community structure, indicating either less favorable growth conditions for the pathogen in higher regions or favorable conditions for co-occurring taxa. This emphasizes that taxonomic identification of co-occurring fungi and their ecological relevance (e.g., potential mycoparasites) during CLR infection requires further research. This study provides a foundational framework for global coffee research by emphasizing the untapped potential of fungal community analyses to develop innovative, microbiome-informed strategies for managing coffee leaf rust and improving crop resilience
dc.format12 pag
dc.formatapplication/pdf
dc.identifierhttps://repositorio.catie.ac.cr/handle/11554/14292
dc.identifieropenAccess
dc.identifier.urihttp://hdl.handle.net/123456789/1387
dc.languageen
dc.subjectPlaguicida microbiano||microbial pesticides||pesticida microbiano||pesticide microbien
dc.subjectMicrobioma||microbiomes||undefined||microbiome
dc.subjectCafé||coffee||café||café
dc.subjectCrecimiento||growth||crescimento||croissance
dc.subjectSanidad vegetal||plant health||fitossanidade||santé des plantes
dc.subjectSede Central
dc.subjectODS 12 - Producción y consumo responsables
dc.subjectODS 13 - Acción por el clima
dc.subjectODS 15 - Vida de ecosistemas terrestres
dc.titleFungal diversity associated with coffee leaf rust (Hemileia vastatrix) pustules based on ITS1 amplicon sequencin
dc.typeArtículo

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