Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids

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dc.creatorZhou, Y.
dc.creatorZhang, J.
dc.creatorSun, H.
dc.creatorTao, D.
dc.creatorXu, B.
dc.creatorHan, X.
dc.creatorRen, R.
dc.creatorRuan, J.
dc.creatorSteinaa, Lucilla
dc.creatorHemmink, Johanneke D.
dc.creatorHan Jianlin
dc.creatorLi, X.
dc.creatorXu, J.
dc.creatorZhao, S.
dc.creatorXie, S.
dc.creatorZhao, C.
dc.date2023-10-20
dc.date2023-09-21T11:55:23Z
dc.date2023-09-21T11:55:23Z
dc.date.accessioned2026-06-27T16:43:52Z
dc.descriptionThe development of a contamination-free and on-site nucleic acid detection platform with high sensitivity and specificity but low-cost for the detection of pathogenic nucleic acids is critical for infectious disease diagnosis and surveillance. In this study, we combined the recombinase-aided amplification (RAA) with the exonuclease III (Exo III)-assisted signal amplification into a platform for sensitive and specific detection of nucleic acids of African swine fever virus (ASFV). We found that this platform enabled a naked eye visual detection of ASFV at a detection limit as low as 2 copies/μL in 30 min. As expected, no cross-reactivity was observed with other porcine viruses. In addition, to avoid aerosol contamination, a one-tube RAA-Exo III colorimetric assay was also established for the accurate detection of ASFV in clinical samples. Taken together, we developed a rapid, instrument-free, and low-cost Exo III-assisted RAA colorimetric-assay-based nucleic acid detection platform.
dc.identifierhttps://hdl.handle.net/10568/131922
dc.identifier.urihttp://hdl.handle.net/123456789/131658
dc.languageen
dc.publisherAmerican Chemical Society
dc.rightsLimited Access
dc.sourceZhou, Y., Zhang, J., Sun, H., Tao, D., Xu, B., Han, X., Ren, R., Ruan, J., Steinaa, L., Hemmink, J.D., Han, J., Li, X., Xu, J., Zhao, S., Xie, S. and Zhao, C. 2023. Sensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids. ACS Synthetic Biology 12(10): 2877–2886.
dc.subjectafrican swine fever virus
dc.subjectdiagnostic techniques
dc.titleSensitive and specific exonuclease III-assisted recombinase-aided amplification colorimetric assay for rapid detection of nucleic acids
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)