Determinación del potencial celulolítico de bacterias asociadas a compost de estiércol bovino y pasto

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dc.audienceInvestigador
dc.audienceProfesional
dc.contributorRojas Tapias, Daniel Fernando
dc.contributorEstrada Bonilla, Germán Andrés
dc.coverageC.I Tibaitatá
dc.coverageColombia
dc.creatorSantofimio Varón, María Alejandra
dc.date2024-01-15T17:48:58Z
dc.date2024-01-15T17:48:58Z
dc.date2023
dc.date2023
dc.date.accessioned2026-06-27T04:49:34Z
dc.descriptionEn este estudio, se determinó el potencial celulolítico de cepas bacterianas asociadas al compost de estiércol bovino. Para esto, se estableció un método de cribado para la identificación de cepas bacterianas con actividad celulolítica basado en el uso de rojo congo. Se evaluó de manera semicuantitativa la actividad celulasa de 49 cepas termotolerantes provenientes de la Colección de Trabajo del Banco de Microorganismos de Sistemas Agropecuarios Sostenibles. Estas cepas fueron aisladas del compostaje de estiércol bovino y pasto. Para esto, se calcularon los índices celulolíticos basados en halos de degradación de carboximeticelulosa revelados con rojo congo y una solución salina. De igual forma, la evaluación cuantitativa de la actividad celulasa de dieciséis cepas termotolerantes de la colección, que obtuvieron un índice celulolítico mayor a 65% y 14 nuevas cepas termófilas, fue determinada de dos formas. En la primera, se estimó los azúcares formados en caldo 1% carboximetilcelulosa después de 48 horas de incubación. Finalmente, 27 cepas de la colección se caracterizaron basados en el 16S rRNA. El 74.04% de estas cepas se identificó como pertenecientes al género Bacillus, el 10.11% al género Ralstonia, el 7.40% al género Undibacterium, y el resto correspondieron a los géneros Klebsiella, Zeimonas y Priestia cada uno con 3.70%.
dc.descriptionBióloga
dc.descriptionPregrado
dc.format82
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.identifierhttp://hdl.handle.net/20.500.12324/38724
dc.identifierreponame:Biblioteca Digital Agropecuaria de Colombia
dc.identifierinstname:Corporación colombiana de investigación agropecuaria AGROSAVIA
dc.identifier.urihttp://hdl.handle.net/123456789/37280
dc.languagespa
dc.publisherUniversidad del Bosque
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dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectGanadería - L01
dc.subjectEnfermedades de los animales - L73
dc.subjectBacteria
dc.subjectCompost
dc.subjectAzúcares reductores
dc.subjectCelulolisis
dc.subjectEstiércol
dc.subjectGanadería y especies menores
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_765
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_1795
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_32034
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_27481
dc.subjecthttp://aims.fao.org/aos/agrovoc/c_2810
dc.thumbnailhttps://repository.agrosavia.co/bitstreams/6a5effba-f777-4fce-9670-681773f5a885/download
dc.titleDeterminación del potencial celulolítico de bacterias asociadas a compost de estiércol bovino y pasto
dc.typeTrabajo de grado

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