Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties.

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dc.creatorWebi, E.N.
dc.creatorKariuki, D.
dc.creatorKinyua, J.
dc.creatorNjoroge, A.W.
dc.creatorGhislain, M.
dc.creatorMagembe, E.
dc.date2019-09
dc.date2020-02-19T01:05:14Z
dc.date2020-02-19T01:05:14Z
dc.date.accessioned2026-06-27T17:56:18Z
dc.descriptionThree late blight resistance (R) genes from wild potato species were transferred as a stack into the farmer-preferred varieties ‘Tigoni’ and ‘Shangi’. Transgenic events were tested by detached-leaf assays (DLA) and whole-plant assays (WPA) with isolates of Phytophthora infestans using 20,000 sporangia / mL inoculum. For the first DLA, 9 out of 13 ‘Tigoni’ and 10 out of 12 ‘Shangi’ transgenic events had scores below 3% of leaf affected area. 17 of the 19 transgenic events with low scores were subjected to a second DLA using two different P. infestans isolates. 8 of them exhibited extreme LB resistance while the remaining 9 transgenic events showed hypersensitive response (HR). 6 transgenic events (2 ‘Tigoni’ and 4 ‘Shangi’) with extreme resistance by DLA were subjected to WPA and they all exhibited extreme resistance to LB. Hence, this study demonstrates that a simple DLA predicts high level of resistance to late blight. R gene expression analysis in 18 transgenic events showed different transgenic events exhibiting different levels of expression in the three genes. However, that pathogen induction and / or high R gene expression are necessary for extreme resistance when transgenic events bear a stack of three R genes was not observed.
dc.identifierhttps://hdl.handle.net/10568/107143
dc.identifier.urihttp://hdl.handle.net/123456789/149925
dc.languageen
dc.publisherAcademic Journals
dc.rightsOpen Access
dc.sourceWebi, E. N.; Kariuki, D.; Kinyua, J.; Njoroge, A.; Ghislain, M.; Magembe, E. 2019. Extreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties. African Journal of Biotechnology. ISSN: 1684-5315. 18(29). pp. 845-856.
dc.subjectphytophthora infestans
dc.subjectgenes
dc.subjectpotatoes
dc.subjectdisease resistance
dc.titleExtreme resistance to late blight disease by transferring 3 R genes from wild relatives into African farmer-preferred potato varieties.
dc.typeJournal Article

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