Genome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP).

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dc.contributorGUILHERME SOUZA PRADO, especialista visitante CNPAF; THAÍSA TESSUTTI PINHEIRO, especialista visitante CNPAF; JOSIAS CORREA DE FARIA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
dc.creatorPRADO, G. S.
dc.creatorPINHEIRO, T. T.
dc.creatorFARIA, J. C. de
dc.creatorVIANELLO, R. P.
dc.date2021-05-25T02:23:10Z
dc.date2021-05-25T02:23:10Z
dc.date2021-05-24
dc.date2021
dc.date.accessioned2026-07-01T00:05:55Z
dc.descriptionWe will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments.
dc.formatil.
dc.formatp. 47-88.
dc.identifierIn: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021.
dc.identifier978-65-87380-10-0
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1131989
dc.identifier.urihttp://hdl.handle.net/123456789/397304
dc.languageeng
dc.rightsopenAccess
dc.subjectCRISPR
dc.subjectCRISPRevolution
dc.subjectNon-homologous
dc.subjectBiotecnologia
dc.subjectDNA
dc.subjectRNA
dc.subjectEngenharia Genética
dc.subjectGenoma
dc.subjectBiotechnology
dc.subjectGenetic engineering
dc.subjectNucleases
dc.subjectPlant genetics
dc.subjectRibonucleoproteins
dc.subjectRNA editing
dc.titleGenome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP).
dc.titleTecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura, 2020.
dc.typeParte de livro

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