Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses

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dc.creatorBömer, M.
dc.creatorRathnayake, A.I.
dc.creatorVisendi, P.
dc.creatorSewe, S.O.
dc.creatorSicat, J.P.A.
dc.creatorSilva, G.
dc.creatorKumar, P. Lava
dc.creatorSeal, S.E.
dc.date2018-06-19
dc.date2018-07-12T16:09:14Z
dc.date2018-07-12T16:09:14Z
dc.date.accessioned2026-06-27T16:04:12Z
dc.descriptionIn vitro culture offers many advantages for yam germplasm conservation, propagation and international distribution. However, low virus titres in the generated tissues pose a challenge for reliable virus detection, which makes it difficult to ensure that planting material is virus-free. In this study, we evaluated next-generation sequencing (NGS) for virus detection following yam propagation using a robust tissue culture methodology. We detected and assembled the genomes of novel isolates of already characterised viral species of the genera Badnavirus and Potyvirus, confirming the utility of NGS in diagnosing yam viruses and contributing towards the safe distribution of germplasm.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/96123
dc.identifier.urihttp://hdl.handle.net/123456789/121621
dc.languageen
dc.publisherElsevier
dc.rightsOpen Access
dc.sourceBömer, M., Rathnayake, A.I., Visendi, P., Sewe, S.O., Sicat, J.P.A., Silva, G., ... & Seal, S.E. (2018). Tissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses. Physiological and Molecular Plant Pathology, 1-13.
dc.subjectin vitro culture
dc.subjectrna sequence
dc.subjectdioscorea
dc.subjectmosaic virus
dc.subjecttissue culture
dc.subjectyams
dc.subjectvirus free plants
dc.titleTissue culture and next-generation sequencing: a combined approach for detecting yam (Dioscorea spp.) viruses
dc.typeJournal Article

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Instituto Internacional de Agricultura Tropical (IITA)