An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs

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dc.creatorGuild, Georgia E.
dc.creatorPaltridge, Nicholas G.
dc.creatorAndersson, Meike S.
dc.creatorStangoulis, James C.R.
dc.date2017-10
dc.date2017-08-15T18:39:09Z
dc.date2017-08-15T18:39:09Z
dc.date.accessioned2026-06-27T14:39:16Z
dc.descriptionBackground and aims Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea. Methods Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method. Results Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg−1 for both Fe and Zn. Conclusion EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea.
dc.identifierhttps://hdl.handle.net/10568/83153
dc.identifier.urihttp://hdl.handle.net/123456789/84511
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceGuild, Georgia E.; Paltridge, Nicholas G.; Andersson, Meike S.; Stangoulis, James C. R.. 2017. An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs . Plant and Soil 419(1-2): 457-466.
dc.subjectfood enrichment
dc.subjectfood fortification
dc.subjectbiofortied crops
dc.subjectmicronutrients
dc.subjectbreeding
dc.subjectplant
dc.subjectenriquecimiento de los alimentos
dc.subjectfortificación de alimentos
dc.subjectmicronutrientes
dc.subjectmejora
dc.titleAn energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs
dc.typeJournal Article

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