Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
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Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR.
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Colossoma Macropomum, Tambaqui, aquaculture, molecular biology