A protocol for detection of large chromosome variations in Banana using next generation sequencing

Vista sencilla de ítem
dc.creatorBreton, Catherine
dc.creatorCenci, Alberto
dc.creatorSardos, Julie
dc.creatorChase, Rachel
dc.creatorRuas, Max
dc.creatorRouard, Mathieu
dc.creatorRoux, Nicolas
dc.date2022
dc.date2022-06-07T07:41:10Z
dc.date2022-06-07T07:41:10Z
dc.date.accessioned2026-06-27T13:20:53Z
dc.descriptionCore activities of genebank operations include the preservation of germplasm identity and maintenance of genetic integrity. Some organisms such as banana are maintained by tissue culture that can foster accumulation of somatic mutations and loss of genetic integrity. Such changes can be reflected in their genome structure and thus be revealed by sequencing methods. Here, we propose a protocol for the detection of large chromosomal gains and/or losses that was applied to in vitro banana accessions with different levels of ploidy. Mixoploidy was detected in triploid (3x) accessions with chromosomal regions being diploid (2x) and tetraploid (4x) and in diploid accessions (2x) where large deletions resulted in partial haploidy (1x). Such abnormal molecular karyotypes can potentially explain phenotypic aberrations observed in off type material. With the affordable cost of Next Generation Sequencing (NGS) technologies and the release of the presented bioinformatic pipeline, we aim to promote the application of this methodology as a routine operation for genebank management as an important step to monitor the genetic integrity of distributed material. Moreover, genebank users can be also empowered to apply the methodology and check the molecular karyotype of the ordered material.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/119749
dc.identifier.urihttp://hdl.handle.net/123456789/56502
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceBreton, C.; Cenci, A.; Sardos, J.; Chase, R.; Ruas, M.; Rouard, M.; Roux, N. (2022) A protocol for detection of large chromosome variations in Banana using next generation sequencing. In: Jankowicz-Cieslak, J.; Ingelbrecht, I.L. (eds) Efficient screening techniques to identify mutants with TR4 resistance in banana. Berlin, Heidelberg (Germany): Springer. p. 129–148. ISBN: 978-3-662-64914-5
dc.subjectaneuploidy
dc.subjectsomaclonal variation
dc.subjectgene banks
dc.subjectmutation
dc.subjectaneuploidia
dc.subjectvariación somaclonal
dc.subjectbanco de genes
dc.titleA protocol for detection of large chromosome variations in Banana using next generation sequencing
dc.typeBook Chapter

Archivos

Colecciones

Alianza Bioversity International y CIAT