Soybean leaf infusion culture media and phytotoxic metabolite production in Macrophomina phaseolina

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dc.creatorJazmín Vaceque-Acosta
dc.creatorJavier E. Barúa
dc.creatorDani Daniel Ruiz-Diaz-Mendoza
dc.creatorM. Cristina Romero-Rodríguez
dc.creatorAntonio Macías-Sánchez
dc.creatorMaría Eugenia Flores-Giubi
dc.date2025
dc.date.accessioned2026-07-07T03:03:33Z
dc.descriptionIntroduction. The charcoal rot fungus, Macrophomina phaseolina, is a ubiquitous necrotrophic phytopathogen that infecting soybean and other plant species. Despite its significant impact on crops, limited progress has been made in understanding the factors that influence phytotoxic molecule secretion by this phytopathogen. Objective. To evaluate the effect of soybean leaf infusion in the culture medium on the differential secretion of phytotoxic molecules of M. phaseolina. Materials and methods. The study was conducted between 2016 and 2023 at the Departamento de Química Biológica, Universidad Nacional de Asunción, Paraguay. Two fungal isolates were cultured in vitro using potato dextrose broth (PDB) and Czapek-Dox broth media, with or without soybean leaf infusion. Phytotoxic activity of secreted molecules was a using soybean leaf discs. The crude organic extract from the cultures was separated using chromatographic techniques, and purified metabolites were characterized by UHPLC-PDA/MS, HRMS (APGC), HRMSESI, 1HNMR and 13CNMR. Results. Molecules secreted by M. phaseolina FCQ11 cultured in infusion-enriched PDB induced the highest percentage of necrosis. Under these conditions, three differentially secreted metabolites were isolated and identified: (R)-mellein, (3R,4R)-hydroxymellein, and (-)-botryodiplodin. Conclusions. Soybean leaf infusion presence in M. phaseolina growth media stimulates phytotoxic metabolite production and alters the profile of secreted metabolites.
dc.formatapplication/pdf
dc.identifier2215-3608
dc.identifierhttps://www.redalyc.org/articulo.oa?id=43780398050
dc.identifierhttps://www.redalyc.org/journal/437/43780398050/
dc.identifierhttps://www.redalyc.org/journal/437/43780398050/html/
dc.identifierhttps://www.redalyc.org/journal/437/43780398050/43780398050.epub
dc.identifierhttps://www.redalyc.org/journal/437/43780398050/movil
dc.identifier10.15517/x1y07135
dc.identifier.urihttp://hdl.handle.net/123456789/426175
dc.languageen
dc.publisherUniversidad de Costa Rica
dc.relationhttp://www.redalyc.org/revista.oa?id=437
dc.rightsAgronomía Mesoamericana
dc.sourceAgronomía Mesoamericana (Costa Rica) Vol.36
dc.subjectAgrociencias
dc.titleSoybean leaf infusion culture media and phytotoxic metabolite production in Macrophomina phaseolina
dc.typeartículo científico

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Agronomía Mesoamericana - Universidad de Costa Rica