Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae).

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dc.contributorKarina Peres Gramacho, CEPLAC; Júlio Cézar Mattos Cascardo, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; Fabienne Micheli, UESC.
dc.creatorLOPES, M. A.
dc.creatorHORA JUNIOR, B. T.
dc.creatorDIAS, C. V.
dc.creatorSANTOS, G. C.
dc.creatorGRAMACHO, K. P.
dc.creatorCASCARDO, J. C. M.
dc.creatorGESTEIRA, A. da S.
dc.creatorMICHELI, F.
dc.date2022-08-18T13:19:38Z
dc.date2022-08-18T13:19:38Z
dc.date2010-07-28
dc.date2010
dc.date.accessioned2026-07-07T04:51:06Z
dc.descriptionCacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches? broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regulators of cellular processes and are important for the fine-tuning of plant defense responses. We developed a macroarray with 88 TF cDNA from previously obtained cacao-M. perniciosa interaction libraries. Seventy-two TFs were found differentially expressed between the susceptible (Catongo) and resistant (TSH1188) genotypes and/or during the disease time course - from 24 h to 30 days after infection. Most of the differentially expressed TFs belonged to the bZIP, MYB and WRKY families and presented opposite expression patterns in susceptible and resistant cacao-M. perniciosa interactions (i.e., up-regulated in Catongo and down-regulated in TSH1188). The results of the macroarray were confirmed for bZIP and WRKY TFs by real-time PCR. These differentially expressed TFs are good candidates for subsequent functional analysis as well as for plant engineering. Some of these TFs could also be localized on the cacao reference map related to witches? broom resistance, facilitating the breeding and selection of resistant cacao trees.
dc.identifierGenetics and Molecualr Reseach, Ribeirão Preto, v. 9, n. 3, p. 1279-1297, 2010.
dc.identifier1676-5680
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/858659
dc.identifier10.4238/vol9-3gmr825
dc.identifier.urihttp://hdl.handle.net/123456789/473318
dc.languageeng
dc.rightsopenAccess
dc.subjectMacroarrays
dc.subjectRT-qPCR
dc.subjectGene regulation
dc.subjectTheobrama cacao
dc.subjectWitches broom disease
dc.subjectCacau
dc.subjectDoença de Planta
dc.subjectFungo
dc.subjectGenótipo
dc.subjectHibridação
dc.subjectVassoura de Bruxa
dc.titleExpression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae).
dc.typeArtigo de periódico

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