Effect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.

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dc.contributorVANÚZIA GONÇALVES MENEZES, UNIVASF
dc.contributorALANE PAINS OLIVEIRA DO MONTE, UNIVASF
dc.contributorRICÁSSIO DE SOUSA BARBERINO, UNIVASF
dc.contributorBRUNA BORTOLONI GOUVEIA, UNIVASF
dc.contributorTHAE LANE BARBOSA GAMA LINS, UNIVASF
dc.contributorTAÍS JOBARD SILVA E MACEDO, UNIVASF
dc.contributorJOÃOZITO LIANDRO DE OLIVEIRA JÚNIOR, UNIVASF
dc.contributorCLEYTON DE ALMEIDA ARAÚJO, Universidade Federal do Agreste Pernambucano, Garanhuns, PE
dc.contributorFLEMING SENA CAMPOS, Universidade Federal do Maranh ̃ao - UFMA, Chapadina, MA
dc.contributorEMANUEL FELIPE DE OLIVEIRA FILHO, UFRPE
dc.contributorANDRÉ MARIANO BATISTA, UFRPE
dc.contributorMARIO ADRIANO ÁVILA QUEIROZ, UNIVASF
dc.contributorGHERMAN GARCIA LEAL DE ARAUJO, CPATSA
dc.contributorMARIA HELENA TAVARES DE MATOS, UNIVASF
dc.contributorAUREA WISCHRA, UFRPE.
dc.creatorMENEZES, V. G.
dc.creatorMONTE, A. P. O.
dc.creatorBARBERINO, R. de S.
dc.creatorGOUVEIA, B. B.
dc.creatorLINS, T. L. B.
dc.creatorMACEDO, T. J. S. e
dc.creatorOLIVEIRA JÚNIOR, J. L. de
dc.creatorARAÚJO, C. de A.
dc.creatorCAMPOS, F. S.
dc.creatorOLIVEIRA FILHO, E. F. de
dc.creatorBATISTA, A. M.
dc.creatorQUEIROZ, M. A. A.
dc.creatorARAUJO, G. G. L. de
dc.creatorMATOS, M. H. T. de
dc.creatorWISCHRA, A.
dc.date2023-09-19T17:24:21Z
dc.date2023-09-19T17:24:21Z
dc.date2023-09-19
dc.date2023
dc.date.accessioned2026-07-07T05:58:12Z
dc.descriptionThis study evaluated the effect of reduced water intake on survival, apoptosis and immunoexpression of leptin in sheep preantral follicles, activation of primordial follicles, serum levels of leptin, estradiol (E2) and progesterone (P4), and in vitro maturation (IVM) of oocytes antral follicles, as well evaluated the effects of leptin on in vitro culture of secondary follicles isolated these animals. Ewes (n = 32) were divided into four groups: water ad libitum (Control ? 100%), 80%; 60% and 40% of ad libitum intake. Blood was collected to determine, leptin, E2 and P4, before and after experiment. After the slaughter, ovarian cortex was used to histological and immunohistochemistry analysis and oocytes IVM. Moreover, isolated secondary follicles were cultured in vitro for 12 days in control medium (&#945;-MEM+) or &#945;-MEM+ with 10 or 25 ng/mL leptin. The reduction of water intake caused a linear decreasing effect on the percentages of normal preantral follicles, especially of primordial (P < 0.05), increased the apoptosis (P < 0.05) and decreased leptin expression in preantral follicles. The treatment with 60% of water intake showed greater total growth rate of isolated secondary follicles cultured with 25 ng/L leptin (P < 0.05), compared to those cultured in &#945;-MEM+ . In conclusion, reduced water intake impaired the number of normal sheep preantral follicles, especially of primordial follicles, increased apoptosis and decreased leptin expression in preantral follicles. Moreover, secondary follicles from of ewes
dc.identifierAnimal Reproduction Science, v. 255, 107278, Jun. 2023.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1156781
dc.identifierhttps://doi.org/10.1016/j.anireprosci.2023.107278
dc.identifier.urihttp://hdl.handle.net/123456789/503592
dc.languageeng
dc.rightsopenAccess
dc.subjectFoliculogênese
dc.subjectFolículos primordiais
dc.subjectOócito
dc.subjectHormônios sexuais
dc.subjectOvelha
dc.subjectÁgua Potável
dc.subjectDrinking water
dc.subjectOocytes
dc.subjectSex hormones
dc.subjectSheep
dc.titleEffect of reduced water intake on ovarian reserve, leptin immunoexpression and impact of leptin on the in vitro culture of sheep secondary follicles.
dc.typeArtigo de periódico

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