Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern

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dc.creatorHaile, A.F.
dc.creatorAlonso, Silvia
dc.creatorBerhe, N.
dc.creatorAtoma, T.B.
dc.creatorBoyaka, P.N.
dc.creatorGrace, Delia
dc.date2021-07-16
dc.date2021-07-19T11:54:30Z
dc.date2021-07-19T11:54:30Z
dc.date.accessioned2026-06-27T16:44:58Z
dc.descriptionEscherichia coli O157:H7 is an important foodborne pathogen but largely under investigated in Africa. The objectives of this study were to estimate the prevalence and pattern of antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. A total of 390 retail lettuce samples were collected across the 10 subcities of Addis Ababa. E. coli O157:H7 was isolated and identified following ISO-16654:2001 standard. The isolates were further tested for antimicrobial susceptibility to 13 antimicrobials using the Kirby–Bauer disk diffusion method. Out of the 390 lettuce samples examined, two (0.51%) carried E. coli O157:H7. The antimicrobial susceptibility pattern of strains showed resistance to ampicillin (100%) and tetracycline (50.0%). One of the two isolates was multidrug resistant to two antimicrobials tested. The results of this study demonstrate the presence of drug-resistant E. coli O157:H7 in lettuce in markets in Addis Ababa. Despite the low prevalence, its presence in a product that is eaten raw highlights potential public health risk in the area associated with this pathogen.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/114326
dc.identifier.urihttp://hdl.handle.net/123456789/132128
dc.languageen
dc.publisherFrontiers Media
dc.rightsOpen Access
dc.sourceHaile, A.F., Alonso, S., Berhe, N., Atoma, T.B., Boyaka, P.N. and Grace, D. 2021. Escherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern. Frontiers in Microbiology 12: 694506.
dc.subjectfood safety
dc.subjectantimicrobial resistance
dc.titleEscherichia coli O157:H7 in retail lettuce (Lactuca sativa) in Addis Ababa city: Magnitude of contamination and antimicrobial susceptibility pattern
dc.typeJournal Article

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