In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages
| dc.creator | HY Lee | |
| dc.creator | D Biswas | |
| dc.creator | J Ahn | |
| dc.date | 2015 | |
| dc.date.accessioned | 2026-07-07T03:23:04Z | |
| dc.description | This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combination treatment with Salmonella and phage (S+P). The number of S. Typhimurium associated on INT-407 cells was reduced from 4.2 to 2.7 log cfu/cm2 by phage treatment (SP). The number of intracellular S. Typhimurium within INT-407 cells was significantly reduced to below the detection limit (0.7 log cfu/cm2) compared with the control (3.4 log cfu/cm2). S. Typhimurium remained inside HD11 cells at 49% and 17% levels in the absence and presence of phages, respectively, at 24 h post-infection (hpi). The expression levels of IFN-g, IL-10, IL-1b, IL-6, IL-8, iNOS, and IL-12 increased in HD11 cells regardless the absence and presence of phages, while those of IL-16, TLR2-1, TLR3, and TLR7 were decreased at 0 and 24 hpi. This study sheds new light on our understanding of the role of phages in Salmonella adhesion, invasion, survival, and cellular immune responses. | |
| dc.format | application/pdf | |
| dc.identifier | 1516-635X | |
| dc.identifier | https://www.redalyc.org/articulo.oa?id=179743242003 | |
| dc.identifier.uri | http://hdl.handle.net/123456789/432207 | |
| dc.language | en | |
| dc.publisher | Fundação APINCO de Ciência e Tecnologia Avícolas | |
| dc.relation | http://www.redalyc.org/revista.oa?id=1797 | |
| dc.rights | Revista Brasileira de Ciência Avícola | |
| dc.source | Revista Brasileira de Ciência Avícola (Brasil) Num.4 Vol.17 | |
| dc.subject | Agrociencias | |
| dc.title | In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages | |
| dc.type | artículo científico |