Community structure of nitrifying and denitrifying bacteria from effluents discharged into Lake Victoria, Kenya

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dc.creatorWachira, J.M.
dc.creatorKiplimo, D.
dc.creatorThuita, M.
dc.creatorMasso, C.
dc.creatorMwirichia, R.
dc.date2022-09
dc.date2022-11-23T09:55:30Z
dc.date2022-11-23T09:55:30Z
dc.date.accessioned2026-06-27T15:50:21Z
dc.descriptionAn active microbial community of nitrifying and denitrifying bacteria is needed for efficient utilization of nitrogenous compounds from wastewater. In this study, we explored the bacterial community diversity and structure within rivers, treated and untreated wastewater treatment plants (WWTPs) discharging into Lake Victoria. Water samples were collected from rivers and WWTPs that drain into Lake Victoria. Physicochemical analysis was done to determine the level of nutrients or pollutant loading in the samples. Total community DNA was extracted, followed by Illumina high throughput sequencing to determine the total microbial community and abundance. Enrichment and isolation were then done to recover potential nitrifiers and denitrifiers. Physicochemical analysis pointed to high levels total nitrogen and ammonia in both treated and untreated WWTPs as compared to the samples from the lake and rivers. A total of 1,763 operational taxonomic units (OTUs) spread across 26 bacterial phyla were observed with the most dominant phylum being Proteobacteria. We observed a decreasing trend in diversity from the lake, rivers to WWTPs. The genus Planktothrix constituted 19% of the sequence reads in sample J2 collected from the lagoon. All the isolates recovered in this study were affiliated to three genera: Pseudomonas, Klebsiella and Enterobacter in the phylum Proteobacteria. A combination of metagenomic analysis and a culture-dependent approach helped us understand the relative abundance as well as potential nitrifiers and denitrifiers present in different samples. The recovered isolates could be used for in situ removal of nitrogenous compounds from contaminated wastewater.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/125650
dc.identifier.urihttp://hdl.handle.net/123456789/114979
dc.languageen
dc.publisherSpringer
dc.rightsOpen Access
dc.sourceWachira, J.M., Kiplimo, D., Thuita, M., Masso, C. & Mwirichia, R. (2022). Community structure of nitrifying and denitrifying bacteria from effluents discharged into Lake Victoria, Kenya. Current Microbiology, 79(9): 252, 1-10.
dc.subjectcommunity
dc.subjectbacterial diseases
dc.subjectkenya
dc.subjectnitrogen fixing bacteria
dc.subjectwastewater treatment
dc.subjectdna
dc.subjectmicrobiology
dc.titleCommunity structure of nitrifying and denitrifying bacteria from effluents discharged into Lake Victoria, Kenya
dc.typeJournal Article

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Instituto Internacional de Agricultura Tropical (IITA)