Cervical relaxation protocol in sheep decreases Oct-4 and Nanog gene expression temporarily.

SANTOS, J. D. R.; BATISTA, R. I. T. P.; TAIRA, A. R.; SOUZA-FABJAN, J. M. G.; ESPÍRITO SANTO, C. G. do; BRAIR, V. L.; MONTEIRO NETTO, M.; BALARO, M. F. A.; FONSECA, J. F. da; BRANDÃO, F. Z.

Cervical relaxation protocol in sheep decreases Oct-4 and Nanog gene expression temporarily.

Autores

SANTOS, J. D. R.

BATISTA, R. I. T. P.

TAIRA, A. R.

SOUZA-FABJAN, J. M. G.

ESPÍRITO SANTO, C. G. do

Descripción

Hormonal protocols aiming at dilation of the sheep cervix were developed to allow access to the uterus. Despite the proven efficacy of these protocols, little is known about these drugs used on embryonic quality. Thus, this study evaluated the influence of the hormonal protocol of cervical dilation (based on estradiol benzoate, d?cloprostenol and oxytocin) on the expression profile of genes related to incidence of apoptosis (Bax and Bcl-2), stress (Hsp90 and PRDX1) and pluripotency maintenance (Oct-4 and NANOG). For this, two experiments were conducted. In conclusion, the cervical relaxation protocol based on the administration of estradiol benzoate, d-cloprostenol and oxytocin may temporarily alter the expression of NANOG and OCT4 genes in surgically recovered sheep embryos.