Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences

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dc.creatorAllsopp, B.A.
dc.creatorBayles, H.A.
dc.creatorAllsopp, M.T.
dc.creatorCavalier-Smith, T.
dc.creatorBishop, Richard P.
dc.creatorCarrington, D.M.
dc.creatorSohanpal, B.K.
dc.creatorSpooner, P.R.
dc.date1993-08
dc.date2013-06-11T09:23:35Z
dc.date2013-06-11T09:23:35Z
dc.date.accessioned2026-06-27T17:19:21Z
dc.descriptionThe complete small subunit ribosomal RNA (srRNA) gene of Theileria parva was cloned and sequenced. Two primers were designed which permitted the specific amplification of part of the Theileria srRNA gene from Theileria-infected cell line samples which were predominantly (> 95%) bovine DNA. The sequence of the central (variable) region of the srRNA genes of T. annulata, T. taurotragi, T. mutans and two unidentified parasites referred to as Theileria sp. (buffalo) and Theileria sp. (Marula) were obtained. An alignment of the sequences was generated from which 6 oligonucleotide probes, corresponding to species-specific regions, were designed. These probes were demonstrated to provide unequivocal identification of each of the 6 species either by direct detection of parasite srRNA or by hybridization to amplified parasite srRNA genes. The probes were not able to distinguish buffalo-derived T. parva, the causal agent of Corridor disease, from cattle-derived T. parva, the causal agent of East Coast fever.
dc.identifierhttps://hdl.handle.net/10568/29443
dc.identifier.urihttp://hdl.handle.net/123456789/148817
dc.languageen
dc.publisherCambridge University Press
dc.rightsLimited Access
dc.sourceParasitology;107: 157-165
dc.subjecttheileria
dc.subjectmetastigmata
dc.subjectnucleotides
dc.subjectdna
dc.subjectanimal diseases
dc.titleDiscrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)