Pathological, Immunohistochemical, and Molecular Findings in Commercial Laying Hens and in Backyard Chickens Naturally Infected with the Infectious Laryngotracheitis Virus

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dc.creatorIS Preis
dc.creatorATL Fiúza
dc.creatorCC Silva
dc.creatorJFV Braga
dc.creatorRM Couto
dc.creatorNR da S Martins
dc.creatorR Ecco
dc.date2014
dc.date.accessioned2026-07-07T03:25:04Z
dc.descriptionSeventy-eight chickens from a very high poultry density (approximately eight million) region and twelve backyard chickens from neighboring areas were analyzed by histopathology and additional techniques for the presence of the infectious laryngotracheitis virus. The virus distribution was determined in different tissues using immunohistochemistry (IHC) and polymerase chain reaction (PCR). The disease was histopathologically diagnosed in 41.0% (32/78) of the commercial layers. Lesions were mainly characterized by syncytial cells with eosinophilic intranuclear inclusion body formed from the hyperplastic epithelium of the upper respiratory tract, primary and secondary bronchi, and conjunctiva. IHC showed 70% (21/30) positive signal in the larynx/trachea and, 53.8% (14/26) in the lungs, either in epithelial cells or syncytia. In the turbinates and paranasal sinuses, 29.6% (8/27) of samples showed positive signal. PCR detected the following gallid herpesvirus 1-positive percentages: conjunctiva 63.2% (31/49), lungs 57.6% (30/52), turbinates and paranasal sinuses 56% (28/50), and larynx/trachea 50% (39/78). IHC showed to be a useful additional tool for definitive ILT diagnosis, especially during the subacute phase of the disease when syncytial cells with intranuclear inclusion bodies are no longer observed. PCR using specific primers from ICP4 gene, generating a product of 237 base pairs, was sensitive for ILT diagnosis, and very useful for rapid detection of GaHV-1 in chickens. Fixed tissues allowing histopatological examination and detection of GaHV-1 by PCR, are a good option in areas where farms are located several hundred kilometers away from a diagnostic center, reducing problems with conservation of fresh samples and the risk of virus spread.
dc.formatapplication/pdf
dc.identifier1516-635X
dc.identifierhttps://www.redalyc.org/articulo.oa?id=179732914004
dc.identifier.urihttp://hdl.handle.net/123456789/433444
dc.languageen
dc.publisherFundação APINCO de Ciência e Tecnologia Avícolas
dc.relationhttp://www.redalyc.org/revista.oa?id=1797
dc.rightsRevista Brasileira de Ciência Avícola
dc.sourceRevista Brasileira de Ciência Avícola (Brasil) Num.4 Vol.16
dc.subjectAgrociencias
dc.titlePathological, Immunohistochemical, and Molecular Findings in Commercial Laying Hens and in Backyard Chickens Naturally Infected with the Infectious Laryngotracheitis Virus
dc.typeartículo científico

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Revista Brasileira de Ciência Avícola - Fundação APINCO de Ciência e Tecnologia Avícolas