Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities

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dc.creatorWebi, Ethel
dc.creatorAbkallo, Hussein M.
dc.creatorObiero, G.
dc.creatorNdegwa, P.
dc.creatorXie, S.
dc.creatorZhao, S.
dc.creatorNene, Vishvanath
dc.creatorSteinaa, Lucilla
dc.date2024-12-01
dc.date2024-10-17T10:28:05Z
dc.date2024-10-17T10:28:05Z
dc.date.accessioned2026-06-27T16:44:42Z
dc.descriptionClustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) technology has revolutionized genome editing across various biological systems, including the Apicomplexa phylum. This review describes the status, challenges, and applications of CRISPR–Cas9 editing technology in apicomplexan parasites, such as <i>Plasmodium</i>, <i>Toxoplasma</i>, <i>Theileria</i>, <i>Babesia</i>, and <i>Cryptosporidium</i>. The discussion encompasses successfully implemented CRISPR–Cas9-based techniques in these parasites, highlighting the achieved milestones, from precise gene modifications to genome-wide screening. In addition, the review addresses the challenges hampering efficient genome editing, including the parasites’ complex life cycles, multiple intracellular stages, and the lack of robust genetic tools. It further explores the ethical and policy considerations surrounding genome editing and the future perspectives of CRISPR–Cas applications in apicomplexan parasites.
dc.identifierhttps://hdl.handle.net/10568/155388
dc.identifier.urihttp://hdl.handle.net/123456789/132009
dc.languageen
dc.publisherMary Ann Liebert Inc
dc.rightsOpen Access
dc.sourceWebi, E., Abkallo, H.M., Obiero, G., Ndegwa, P., Xie, S., Zhao, S., Nene, V. and Steinaa, L. 2024. Genome editing in apicomplexan parasites: Current status, challenges, and future possibilities. CRISPR Journal 7(6): 310–326.
dc.subjectcrispr
dc.subjectgenomics
dc.titleGenome editing in apicomplexan parasites: Current status, challenges, and future possibilities
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)