Prevalence and morphological appearance of sperm nuclear defects using feulgen reaction in beef Bulls.

Vista sencilla de ítem
dc.contributorCARLOS E. FERNANDES, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; ADRIANE L. ZART, PERSONAL PEC; JUAN CUEVAS DE ALVARENGA MARTINS, UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL; RICHARD OKO, QUEEN'S UNIVERSITY; PETER SUTOVSKY, UNIVERSITY OF MISSOURI; ERIKLIS NOGUEIRA, CNPGC.
dc.creatorFERNANDES, C. E.
dc.creatorZART, A. L.
dc.creatorMARTINS, J. C. de A.
dc.creatorOKO, R.
dc.creatorSUTOVSKY, P.
dc.creatorNOGUEIRA, E.
dc.date2026-01-26T20:48:42Z
dc.date2026-01-26T20:48:42Z
dc.date2026-01-26
dc.date2025
dc.date.accessioned2026-07-07T03:43:25Z
dc.descriptionABSTRACT - The objective of this study was to assess the prevalence and compare the morphological aspects of sperm nuclei abnormalities in beef bulls submitted to breeding soundness evaluation (BSE). Semen samples were collected by electroejaculation from 649 beef bulls (Bos indicus, n = 515 and Bos indicus × Bos taurus crossbreed, n = 134) ranging from 2 to 12 years of age. Following a clinical evaluation and semen assessment, a slide was prepared with fresh semen and stained by using the Feulgen-stain for the purpose of evaluating sperm and nuclear morphology. Abnormal sperm nuclei were classified into three categories: A, multiple vacuoles in a pouch-like formation; B, a single vacuole such as the nuclear crater/diadem defect; and C, abnormal chromatin condensation. In accordance with the BSE criteria, the animals were classified as either satisfactory or unsatisfactory potential breeders. The prevalence of nuclear defects was comparable between satisfactory bulls of both genotypes. Unsatisfactory Bos indicus bulls exhibited a higher frequency of A and B nuclear defect categories. A category presented an odds ratio of 7.14 (0.012) for unsatisfactory bulls. A low percentage of the C category (2.3%) was observed in both BSE classifications. Vacuoles or craters were predominantly observed in the post-acrosomal and mid-subacrosomal regions, with minimal occurrence in the apical ridge region. Our findings provide the baseline for nuclear defects in B. indicus and its crossbreeds. The Feulgen reaction thus enables a simple yet comprehensive analysis of nuclear sperm head defects during BSE. In addition, stain retention facilitates a practical evaluation and an accurate account of the nuclear lesion types.
dc.identifierReproduction in Domestic Animals, v. 60, e70021, 2025.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1183900
dc.identifierhttps://doi.org/10.1111/rda.70021
dc.identifier.urihttp://hdl.handle.net/123456789/441649
dc.languageeng
dc.rightsopenAccess
dc.subjectBos Indicus
dc.subjectBos Taurus
dc.subjectSêmen
dc.subjectBeef bulls
dc.subjectGenotype
dc.subjectSpermatozoa
dc.titlePrevalence and morphological appearance of sperm nuclear defects using feulgen reaction in beef Bulls.
dc.typeArtigo de periódico

Archivos

Colecciones

Embrapa Ganado Vacuno (CNPGC)