An Improved Protocol for Atnhurium Callus Induction
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Researchers have found that callus growth in many anthurium (Anthurium andreamum Andre) cultivars is too slow and inconsistent for exploitation in large-scale rnicropropagation. Thidiazuron (TDZ) (N-phenyl -N'I ,2,3-thiadiazol- 5-yl-urea, and several substituted pridyl phenyl urea compounds have shown strong cytokininlike effect in a wide range of species including those that have little response to conventional adenine-based cytokinins. We have initiated experiments on the use ofTDZ for in vitro regeneration of anthurium from leaf explants. In readily regenerating anthurium geneotypes using TDZ, the first signs of callus formation were visible from as early as three weeks and whole plants were regenerated and rooted within six months.