Embryo biopsy as a tool for preimplantation identification of beta-lactoglobulin-edited bovine embryos.

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dc.contributorCAROLINA DAVID VIEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; DIANA RANGEL DE LEMOS, UNIVERSIDADE FEDERAL DE VIÇOSA; HYAGO PASSE PEREIRA; ELIZA DINIZ DE SOUZA; NAIARA ZOCCAL SARAIVA, CNPGL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; CLARA SLADE OLIVEIRA, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL.
dc.creatorVIEIRA, C. D.
dc.creatorLEMOS, D. R. de
dc.creatorPEREIRA, H. P.
dc.creatorSOUZA, E. D. de
dc.creatorSARAIVA, N. Z.
dc.creatorSIQUEIRA, L. G. B.
dc.creatorOLIVEIRA, C. S.
dc.creatorQUINTAO, C. C. R.
dc.creatorCAMARGO, L. S. de A.
dc.date2025-11-24T19:52:40Z
dc.date2025-11-24T19:52:40Z
dc.date2025-11-24
dc.date2025
dc.date.accessioned2026-07-07T04:00:53Z
dc.descriptionGene editing offers opportunities for the genetic improvement of animals. However, mosaicism continues to represent a significant challenge in genetic modification experiments, due to the presence of two or more cell lines with distinct genotypes within the same organism, hindering both accurate genotyping and reliable transmission of mutations to offspring. In this context, preimplantation genetic screening of embryos emerges as a promising strategy. In the present study, the CRISPR/Cas9 system combined with electroporation (EP) using a Nepa21 electroporator (Nepagene, Japan) was employed to edit the beta-lactoglobulin (BLG) gene in in vitro-fertilized bovine oocytes. EP of the sgRNA and Cas9 components was carried out 8 hours after fertilization
dc.formatp. 236.
dc.identifierIn: INTERNATIONAL CONGRESS OF THE BRAZILIAN GENETICS SOCIETY, 70., 2025, Belém. E-book: [abstracts]. Ribeirão Preto: Sociedade Brasileira de Genética, 2025.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1181829
dc.identifier.urihttp://hdl.handle.net/123456789/450557
dc.languageeng
dc.rightsopenAccess
dc.subjectEdição genética
dc.subjectBovino
dc.subjectEmbrião Animal
dc.subjectMelhoramento Genético Animal
dc.subjectBiopsia
dc.titleEmbryo biopsy as a tool for preimplantation identification of beta-lactoglobulin-edited bovine embryos.
dc.typeResumo em anais e proceedings

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