Production of Polymeric Membranes Functionalized with Vitreous Humor and Secretome from Dental Pulp Stem Cells for Use in Epithelial Regeneration.

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dc.contributorWALLADY DA SILVA BARROSO, FEDERAL UNIVERSITY OF CEARÁ
dc.contributorERIKA PATRÍCIA CHAGAS GOMES LUZ, FEDERAL UNIVERSITY OF CEARÁ
dc.contributorLIDYANE SOUTO MACIEL MARQUES, FEDERAL UNIVERSITY OF CEARÁ
dc.contributorPAULO EDUARDO DA SILVA CAVALCANTE, FEDERAL UNIVERSITY OF CEARÁ
dc.contributorFRANCISCO FÁBIO PEREIRA DE SOUZA, FEDERAL UNIVERSITY OF CEARÁ
dc.contributorADRIANO LINCOLN ALBUQUERQUE MATTOS, CNPAT
dc.contributorFABIA KARINE ANDRADE, FEDERAL UNIVERSITY OF CEARÁ
dc.contributorANDRÉ LUÍS COELHO DA SILVA, FEDERAL UNIVERSITY OF CEARÁ
dc.contributorMARIÁH CATIONI HIRATA, R-CRIO STEM CELLS
dc.contributorISADORA BOSCO, R-CRIO STEM CELLS
dc.contributorDANIEL NAVARRO DA ROCHA, R-CRIO STEM CELLS
dc.contributorRENATA FRANCIELLE BOMBALDI DE SOUZA, R-CRIO STEM CELLS
dc.contributorFERNANDA CARLA BOMBALDI DE SOUZA, R-CRIO STEM CELLS
dc.contributorJOSÉ RICARDO MUNIZ FERREIRA, R-CRIO STEM CELLS
dc.contributorRODRIGO SILVEIRA VIEIRA, FEDERAL UNIVERSITY OF CEARÁ.
dc.creatorBARROSO, W. da S.
dc.creatorLUZ, E. P. C. G.
dc.creatorMARQUES, L. S. M.
dc.creatorCAVALCANTE, P. E. da S.
dc.creatorSOUZA, F. F. P. de
dc.creatorMATTOS, A. L. A.
dc.creatorANDRADE, F. K.
dc.creatorSILVA, A. L. C. da
dc.creatorHIRATA, M. C.
dc.creatorBOSCO, I.
dc.creatorROCHA, D. N. da
dc.creatorSOUZA, R. F. B. de
dc.creatorSOUZA, F. C. B. de
dc.creatorFERREIRA, J. R. M.
dc.creatorVIEIRA, R. S.
dc.date2026-01-14T13:48:42Z
dc.date2026-01-14T13:48:42Z
dc.date2026-01-14
dc.date2025
dc.date.accessioned2026-06-30T23:05:50Z
dc.descriptionThis study investigated the development of porous membranes based on a matrix of bacterial cellulose (BC) and sodium alginate (ALG) incorporated with proteins extracted from the tuna vitreous humor and complete conditioned medium (CCM) obtained from the in vitro culture of dental pulp stem cells. These bioactive components were integrated into BC-ALG membranes, which were then evaluated for morphology, physicochemical properties, in vitro cytotoxicity, and regenerative potential in vitro. The membranes exhibited high porosity (72% to 89%), and remarkable swelling capacity reaching up to 3376 ± 0.78% in 72 min, indicating excellent moisture retention essential for wound healing in vivo. Cytotoxicity assay showed that none of the formulations were cytotoxic to the tested mammalian cells, confirming their cytocompatibility. Membranes containing CCM showed enhanced cell viability at lower concentrations (500 μg/ mL and 250 μg/mL). In vitro scratch assays demonstrated that membranes with vitreous humor (VH) and CCM reduced scratch closure time from 18 to 12 h, suggesting their potential for accelerating skin regeneration. This work underscores the promise of using sustainable, waste-derived materials for creating bioactive membranes with applications in tissue engineering and regenerative medicine
dc.identifierACS Applied Bio Materials, 8, 7011−7022, 2025.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1183553
dc.identifierhttps://doi.org/10.1021/acsabm.5c00743
dc.identifier.urihttp://hdl.handle.net/123456789/374055
dc.languageeng
dc.rightsopenAccess
dc.subjectBacterial cellulose
dc.subjectVitreous humor
dc.subjectBiological waste
dc.subjectWound dressings
dc.subjectSecretome
dc.titleProduction of Polymeric Membranes Functionalized with Vitreous Humor and Secretome from Dental Pulp Stem Cells for Use in Epithelial Regeneration.
dc.typeArtigo de periódico

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