Antimicrobial and insecticidal protein isolated from seeds of Clitoria ternatea (L.), a tropical forage legume

dc.creatorKelemu, Segenet
dc.creatorCardona, C.
dc.creatorSegura, G.
dc.date2004-12
dc.date2012-07-09T05:53:31Z
dc.date2012-07-09T05:53:31Z
dc.date.accessioned2026-06-27T17:15:15Z
dc.descriptionThe tropical forage legume Clitoria ternatea (L.) has important agronomic traits such as adaptation to a wide range of soil conditions and resistance to drought. It is resistant to a number of pathogens and pests. These important traits gave us reasons to look more closely at the plant. A highly basic small protein was purified from seeds of C. ternatea to homogeneity by using ultrafiltration with Centricon-3 membrane tubes and preparative granulated-bed isoelectric focusing (IEF). A single protein band was obtained on both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and IEF gels. The protein, designated ‘finotin’, has broad and potent inhibitory effect on the growth of various important fungal pathogens of plants, namely Rhizoctonia solani, Fusarium solani, Colletotrichum lindemuthianum, Lasiodiplodia theobromae, Pyricularia grisea, Bipolaris oryzae and Colletotrichum gloeosporioides. It also inhibits the common bean bacterial blight pathogen Xanthomonas axonopodis pv. phaseoli. Moreover, finotin has powerful inhibitory properties against the bean bruchids Zabrotes subfasciatus and Acanthoscelides obtectus.
dc.identifierhttps://hdl.handle.net/10568/21176
dc.identifier.urihttp://hdl.handle.net/123456789/146805
dc.languageen
dc.publisherElsevier
dc.rightsLimited Access
dc.sourceKelemu, S., Cardona, C. and Segura, G. 2004. Antimicrobial and insecticidal protein isolated from seeds of Clitoria ternatea (L.), a tropical forage legume. Plant Physiology and Biochemistry 42(11): 867-873.
dc.subjectfeeds
dc.subjectfeed crops
dc.subjectanimal feeding
dc.titleAntimicrobial and insecticidal protein isolated from seeds of Clitoria ternatea (L.), a tropical forage legume
dc.typeJournal Article

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