Isolation and Identification of Nitrite-oxidizing Bacteria
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In order to select the strain that can degrade nitrite, we use the screening plate with nitrite as the sole nitrogen source to select the strain with ability to degrade nitrite, and get a strain with nitrite degrading capacity from the silt of shrimp farming pond in Hepu City, Guangxi Zhuang Autonomous Region. By identifying the strain from colony morphology, physiological and biochemical characteristics and 16S rRNA sequence, we finally get a bacteria strain that can degrade nitrite, and this strain can grow well on the culture medium with nitrite concentration of 2 g/L. Based on morphology, nitrogen source requirements and evolutionary tree analysis of the above 16S rRNA sequence, it is found that this strain belongs to Pannonibacter phragmitetus. According to the screening location, it is named HPPP007 strain.