Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue

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dc.creatorPerea, Claudia Samantha
dc.creatorHernández, Diana
dc.creatorJauregui, Rosa
dc.date2023-11
dc.date2023-11-28T17:26:28Z
dc.date2023-11-28T17:26:28Z
dc.date.accessioned2026-06-27T13:35:19Z
dc.descriptionThis report presents the detailed protocol that we used for our first attempt in measuring ploidy using fresh leaf tissue in Interspecific Brachiaria using the sysmex flow cytometer with DAPI cystain UV stain. Accurate determination of ploidy is crucial for understanding the genetic characteristics of plant populations, aiding in plant breeding programs, and contributing to advancements in molecular biology. The following methodology outlines the steps for sample collection, preparation, staining, and analysis. This protocol comes from the training in the sysmex flow cytometer on 5/11/202 in CIAT- headquarters by the company representatives. Although preliminary results from the technique are inconclusive, mostly because of technical difficulties with the flow cytometer, this is a first approach to standardize the protocol and it will give us insights in how to proceed next year.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/134766
dc.identifier.urihttp://hdl.handle.net/123456789/64116
dc.languagees
dc.publisherInternational Center for Tropical Agriculture
dc.rightsOpen Access
dc.sourcePerea, C.; Hernández, D.; Jauregui, R. (2023) Measuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Measuring ploidy in Interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue. Cali (Colombia): Centro Internacional de Agricultura Tropical (CIAT). 15 p.
dc.subjectmeasurement
dc.subjectgenetic markers
dc.subjectmarcadores genéticos
dc.subjecturochloa
dc.titleMeasuring ploidy in interspecific Urochloa (syn. Brachiaria) using Sysmex cyflow cytometer with DAPI Cystain UV stain: Protocol for measuring ploidy in Interspecific Brachiaria fresh leaf tissue
dc.titleProtocolo para medir la ploidía en tejido de hojas frescas interespecíficas de Brachiaria
dc.typeReport

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