EFECTO DE UN ANÁLOGO DE BRASINOESTEROIDES (MH5) EN LA PROPAGACIÓN DE Eucalyptus urograndis EN BIORREACTORES DE INMERSIÓN TEMPORAL
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Instituto Nacional de Ciencias Agrícolas
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Temporary Immersion Bioreactors (TIB) are among the current techniques to reach the quick introduction of new varieties in production. Eucalyptus urograndis ( E. grandis Hill ex Maiden x E. urophylla ) plantlets were used to increase explant quality in TIB and study the effect of plantlet sizes on survival rates during acclimatization. The application of low concentrations (0.01 and 0.1 mg.L -1 ) of the brassinosteroid analogue MH5 in culture media during the elongation stage increased plant height, leaf number, fresh and dry weights in TIB. During acclimatization stage, the lowest sized seedlings (1.1-2.0 cm high) reached the highest survival rates (63 %) without statistical differences with 2.1-3.0 cm classification. Growth variables (plant height, leaf number and root number) got the highest values in the smallest plantlets (1.1-2.0 cm), with significant differences just with leaf number. Seemingly, it appeared that size factor had very little effect on the survival of E urograndis seedlings during the acclimatization process, rather than the quality of different organs confirming it.
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