INFLUENCIA DE UN POLÍMERO DE QUITOSANA EN EL CRECIMIENTO Y LA ACTIVIDAD DE ENZIMAS DEFENSIVAS EN TOMATE (Solanum lycopersicum L.)
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Instituto Nacional de Ciencias Agrícolas
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The aim of this work was to evaluate the effect of chitosan in growth and induction of defence responses in tomato ( Solanum lycopersicum L.) . Tomato seeds from the Amalia cultivar, were imbibed 4 hours in chitosan solutions of 0,1, 1,0, 2,5 y 10 g.L -1 . Besides, plants of 21 days were sprayed with chitosan concentrations of 0,1, 1,0 y 2,5 g.L -1 . Root length, stem length, protein concentrations, and the induction of β-1,3 glucanase and phenylalanine ammonia-lyase (PAL) activities were evaluated in several moments. Results showed that the length of the root and the stem were incremented in dependence of chitosan concentration, but not the total dry weighs. The highest increment in PAL activity in relation to control was observed when the seeds were treated at concentration of 0,1 g.L -1 of chitosan, while foliar aspersion treatments did not showed significances differences in this parameter. The seeds treatments with chitosan at concentrations of 2,5 y 10 g.L -1 induced increases in the β -1,3 glucanase activity, but foliar sprays of 0,1g.L -1 showed the highest levels of this enzymatic activity. These results suggested that chitosan induce defensive and growing responses in tomato which depends of concentrations and application manner.
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