BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva

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dc.creatorSvitek, Nicholas
dc.creatorAwino, Elias
dc.creatorNene, Vishvanath M.
dc.creatorSteinaa, Lucilla
dc.date2015-09
dc.date2015-07-09T05:42:09Z
dc.date2015-07-09T05:42:09Z
dc.date.accessioned2026-06-27T16:37:47Z
dc.descriptionWe have recently shown that the BoLA-A18 variant haplotype (BoLA-6*01302) is more prevalent than the BoLA-A18 haplotype (BoLA-6*01301) in a sample of Holstein/Friesian cattle in Kenya. These MHC class I allelic variants differ by a single amino acid polymorphism (Glu97 to Leu97) in the peptide-binding groove. We have previously mapped an 11-mer peptide epitope from the Theileria parva antigen Tp1 (Tp1214–224) that is presented by BoLA-6*01301. Crystal structure data indicates that Glu97 in the MHC molecule plays a role in epitope binding through electro-static interaction with a lysine residue in position 5 of the epitope, which also functions as an additional anchor residue. In contrast to expectations, we demonstrate that the amino acid substitution in BoLA-6*01302 does not divert the CTL response away from Tp1214–224. The two MHC molecules exhibit similar affinity for the Tp1 epitope and can present the epitope to parasite-specific CTLs derived from either BoLA allelic variants. These data confirm that this BoLA polymorphism does not alter Tp1 epitope specificity and that both allelic variants can be used for Tp1 vaccine studies.
dc.identifierhttps://hdl.handle.net/10568/67244
dc.identifier.urihttp://hdl.handle.net/123456789/128642
dc.languageen
dc.publisherElsevier
dc.rightsOpen Access
dc.sourceSvitek, N., Awino, E., Nene, V. and Steinaa, L. 2015. BoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva. Veterinary Immunology and Immunopathology 167(1-2):80–85.
dc.subjecttheileria
dc.subjectcattle
dc.subjectanimal diseases
dc.subjectvaccines
dc.subjectdisease control
dc.subjecteast coast fever
dc.subjectimmunology
dc.titleBoLA-6*01301 and BoLA-6*01302, two allelic variants of the A18 haplotype, present the same epitope from the Tp1 antigen of Theileria parva
dc.typeJournal Article

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