Genetic fingerprinting of Theileria parva using a telomeric DNA probe

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dc.creatorBishop, Richard P.
dc.creatorSpooner, P.R.
dc.creatorSohanpal, B.K.
dc.date1996-02-29
dc.date2013-06-11T09:23:41Z
dc.date2013-06-11T09:23:41Z
dc.date.accessioned2026-06-27T16:45:23Z
dc.descriptionA cloned Theileria parva telomeric DNA sequence, designated pTpUtel, was used to characterize T. parva stocks and clones by hybridization to EcoRI-digested DNA. Eight of the nine T. parva stocks tested were discriminated by the telomeric restriction-fragment-length polymorphisms (RFLOs). Two isolates derived from buffalo 7014 by tick feeding on different occasions were also differentiated using the probe. The probe gave comparable results on purified piroplasm or schizont-infected lymphocyte DNA and did not cross-hybridize with uninfected bovine DNA. The telomeric restriction pattern of a cloned T. parva parasite remained identical after four passages through ticks and cattle. The telomeric sequence therefore represents a useful additional tool for analysis of theileriosis epidemiology.
dc.identifierhttps://hdl.handle.net/10568/29473
dc.identifier.urihttp://hdl.handle.net/123456789/132308
dc.languageen
dc.publisherSpringer
dc.rightsLimited Access
dc.sourceParasitology Research;82: 275-280
dc.subjectdna fingerprinting
dc.subjecttheileria parva
dc.subjectgenetics
dc.titleGenetic fingerprinting of Theileria parva using a telomeric DNA probe
dc.typeJournal Article

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Instituto Internacional de Investigaciones Pecuarias (ILRI)