Exopolysaccharide from Agaricus brasiliensis LPB and its Scale Up Studies in a Stirred Tank Fermenter.

GERN, J. C.; SANTOS, L. F. dos; HABU, S.; NOSEDA, M. D.; LIMA, L. F. O. de; SILVA, A. L. L. da; SOCCOL, V. T.; BRANDAO, H. de M.; SOCCOL, C. R.

Exopolysaccharide from Agaricus brasiliensis LPB and its Scale Up Studies in a Stirred Tank Fermenter.

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Descripción

Background and purpose: Although the activities of polysaccharides such as beta-glucans are well known in Agaricus brasiliensis, there has been little research on their exopolysaccharides and its characterization or scale up studies. The aim of the study was to study the monosaccharide composition of the exopolysaccharide (EPS) produced by Agaricus brasiliensis LPB3 and its production in a stirred tank fermenter. Materials and methods: Agaricus brasiliensis LPB3 was cultured in submerged fermentation in a medium containing glucose, yeast extract, K2HPO4 and MgSO4. Extracellular polysaccharide was precipitated with four volumes of 95% ethanol. For monosaccharide composition, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). Results and conclusions: The exopolysaccharide produced by Agaricus brasiliensis was predominantly composed by mannose (57,7%) and galactose (28,2%) residues. The studies in a stirred tank fermenter kept the productivity obtained in laboratory scale regarding the production of exopolysaccharides (≈ 1200 mg/L). This result can be considered promising since scale up approach can decrease its yield.