Growth ability, microbial activity and susceptibility to antimicrobials of two strains of Pediococcus pentosaceus, candidates to probiotic
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Instituto de Ciencia Animal
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The objective of this study was to evaluate growth ability, antimicrobial activity and susceptibility to antimicrobials of two strains of Pediococcus pentosaceus (LB-12 and LB-25), candidates to probiotic. Four trials were performed with randomized designs and three repetitions. Strain growth in a MRS medium at 37 ºC had a similar performance, and from 6h of culture, they reached the stationary stage. Their growth speeds were around 0.5 h-1 and duplication times were 1.3 h. Both were able to grow and decrease pH of a medium were fructans of Agave fourcroydes were used as energy source, although its concentration decreased regarding the MRS medium. In addition, strains inhibited the growth of evaluated pathogenic bacteria, with a high antimicrobial action of LB-25 against Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028 and Listeria monocytogenes ATCC 1531. Candidates to probiotic were sensitive to G penicillin, florfenicol, chloramphenicol, amoxicillin clavulanic acid and doxycycline. At the same time, were resistant to trimethoprine-sulfamethoxazole, cephalexin, tetracycline, vancomycin, nalidixic acid, oxalinic acid, enrofloxacin and flumequine, streptomycin, kanamycin and gentamicin. It can be concluded that P. pentosaceus LB-12 and LB-25, in vitro, have an antimicrobial activity against pathogens, are susceptible to antimicrobials and have a proper growth ability in a MRS medium, and when fructans are used as energy source, which favors their inclusion in symbiotic and probiotic formulations for animal production.
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