Evidence for molecular evolutionary conservedness of small heat-shock protein sequence in solanaceaeous crops using in silico methods
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Society for Promotion of Horticulture
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Drought and heat contribute to much of the yield decline in agricultural lands all over the world. The basicphysiological responses developed against drought and heat stress overlie each other, as; both these stresses eventuallylead to dehydration of the cell and to osmotic imbalance. To cope with abiotic stresses, it is necessary to understandplant responses to stresses that disturb homeostatic equilibrium at the cellular and molecular level. Although therehas been remarkable progress in this with development of microarray-based expression profiling methods (togetherwith genomic sequence data), understanding on ways to employ these data to engineer plants with improved stress-tolerance is still at a nascent stage. However, these data can be used for discovering genes, functional microsatellitesand regulatory elements using in silico methods. In this context, single nucleotide repeat marker sequences havebeen identified which is associated with small heat-shock protein sequence (sHSP) for heat tolerance in Capsicumannuum. These sHSP sequences have some structural features in common; its characteristic is that it is homologousand highly conserved. These sequences have been analyzed for molecular evolutionary conservedness in solanaceaeouscrops and have been found to have a single nucleotide repeat sequence and a highly conserved sHSP sequence.
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