Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp.
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World Academy of Science, Engineering and Technology
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The genus Azotobacter has been widely used as biofertilizer
due to its significant effects on the stimulation and
promotion of plant growth in various agricultural species of
commercial interest. In order to obtain significantly viable cellular
concentration, a scale-up strategy for a liquid fermentation process
(SmF) with two strains of A. chroococcum (named Ac1 and Ac10)
was validated and adjusted at laboratory and pilot scale. A batch
fermentation process under previously defined conditions was carried
out on a biorreactor Infors®, model Minifors of 3.5 L, which served
as a baseline for this research. For the purpose of increasing process
efficiency, the effect of the reduction of stirring speed was evaluated
in combination with a fed-batch-type fermentation laboratory scale.
To reproduce the efficiency parameters obtained, a scale-up strategy
with geometric and fluid dynamic behavior similarities was
evaluated. According to the analysis of variance, this scale-up
strategy did not have significant effect on cellular concentration and
in laboratory and pilot fermentations (Tukey, p > 0.05). Regarding air
consumption, fermentation process at pilot scale showed a reduction
of 23% versus the baseline. The percentage of reduction related to
energy consumption reduction under laboratory and pilot scale
conditions was 96.9% compared with baseline.
Ministerio de Agricultura y Desarrollo Rural - MADR
Ministerio de Agricultura y Desarrollo Rural - MADR
Palabras clave
Fertilización - F04, Azotobacter, Fermentación, Biofertilizante, Crecimiento de planta, Transversal, http://aims.fao.org/aos/agrovoc/c_752, http://aims.fao.org/aos/agrovoc/c_2855, http://aims.fao.org/aos/agrovoc/c_24975, http://aims.fao.org/aos/agrovoc/c_08842b17