How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters.

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dc.contributorYANE CAROLINE DOS ANJOS BEZERRA, USP; JOÃO PAULO RODRIGUES MARQUES, USP; LILIANE CRISTINA LIBORIO STIPP, USP; LÍSIA BORGES ATTÍLIO, USP; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, USP.
dc.creatorBEZERRA, Y. C. dos A.
dc.creatorMARQUES, J. P. R.
dc.creatorSTIPP, L. C. L.
dc.creatorATTÍLIO, L. B.
dc.creatorASTUA, J. de F.
dc.creatorMOURÃO FILHO, F. de A. A.
dc.date2021-11-17T12:00:36Z
dc.date2021-11-17T12:00:36Z
dc.date2021-11-17
dc.date2021
dc.date.accessioned2026-07-07T04:50:51Z
dc.descriptionNew approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.
dc.identifierRevista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021.
dc.identifier1806-9967
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1136203
dc.identifier.urihttp://hdl.handle.net/123456789/473231
dc.languagepor
dc.rightsopenAccess
dc.subjectFruta Cítrica
dc.titleHow to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters.
dc.typeArtigo de periódico

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