Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmerpreferred cassava cultivars

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dc.creatorNyaboga, E.
dc.creatorNjiru, J.
dc.creatorNguu, E.
dc.creatorGruissem, W.
dc.creatorVanderschuren, H.
dc.creatorTripathi, L.
dc.date2013-12
dc.date2016-08-30T12:46:23Z
dc.date2016-08-30T12:46:23Z
dc.date.accessioned2026-06-27T15:57:27Z
dc.descriptionCassavagenetictransformationcapacityisstillmostlyrestrictedtoadvancedlaboratoriesintheUSA,EuropeandChina;anditsimplementationandmaintenanceinAfricanlaboratorieshasremainedscarce.TheimpactoftransgenictechnologiesforgeneticimprovementofcassavawilldependlargelyonthetransferofsuchcapabilitiestoresearchersinAfrica,wherecassavahasanimportantsocioeconomicniche.Amajorconstrainttothedevelopmentofgenetictransformationtechnologiesforcassavaimprovementhasbeenthelackofanefficientandrobusttransformationandregenerationsystem.Despitethesuccessachievedingeneticmodificationoffewcassavacultivars,includingthemodelcultivar60444,transgeniccassavaproductionremainsdifficultforfarmer-preferredcultivars.Inthisstudy,aprotocolforcultivar60444developedatETHZurichwassuccessfullyimplementedandoptimizedtoestablishtransformationoffarmer-preferredcassavacultivarspopularineastAfrica.Theconditionsforproductionandproliferationoffriableembryogeniccalli(FEC)andAgrobacterium-mediatedtransformationwereoptimizedforthreeeastAfricanfarmer-preferredcultivars(Ebwanatereka,KibandamenoandSerere).Ourresultsdemonstratedtransformationefficienciesofabout14–22independenttransgeniclinesper100mgofFECforfarmer-preferredcultivarsincomparisonto28linesper100mgofthemodelcultivar60444.Thepresence,integrationandexpressionofthetransgeneswereconfirmedbyPCR,SouthernblotanalysisandhistochemicalGUSassay.ThisstudyreportstheestablishmentofacassavatransformationplatformatInternationalInstituteofTropicalAgriculture(IITA)hostedbyBioscienceseasternandcentralAfrica(BecA)hubinKenyaandprovidesthebasisfortransferringimportanttraitssuchasvirusresistanceandprolongedshelf-lifetofarmer-preferredcultivarsineastAfrica.Weanticipatethatsuchplatformwillalsobeinstrumentaltotransfertechnologiestonationalagriculturalresearchsystems(NARS)insub-SaharanAfrica.
dc.identifierhttps://hdl.handle.net/10568/76672
dc.identifier.urihttp://hdl.handle.net/123456789/118505
dc.languageen
dc.publisherFrontiers Media
dc.rightsOpen Access
dc.sourceNyaboga, E., Njiru, J., Nguu, E., Gruissem, W., Vanderschuren, H. & Tripathi, L. (2013). Unlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmer-preferred cassava cultivars. Frontiers in Plant Science, 4(526), 1-11.
dc.subjectcassava
dc.subjectgenetic transformation
dc.subjectagrobacterium tumefaciens
dc.subjectcultivars
dc.titleUnlocking the potential of tropical root crop biotechnology in east Africa by establishing a genetic transformation platform for local farmerpreferred cassava cultivars
dc.typeJournal Article

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Instituto Internacional de Agricultura Tropical (IITA)