Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans

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dc.creatorAnitha, S.
dc.creatorWaliyar, Farid
dc.creatorReddy, A.S.
dc.creatorRao, R.
dc.creatorKumar, P. Lava
dc.date2011-10-10
dc.date2017-08-28T14:03:31Z
dc.date2017-08-28T14:03:31Z
dc.date.accessioned2026-06-27T14:52:29Z
dc.descriptionIn the present study, high titred polyclonal antibodies were produced against synthetic AFB1-lys and developed a simple indirect competitive (IC)-ELISA for quantitative estimation of AFB1-lys adducts in human serum albumin. IC-ELISA was validated by testing 250 blood samples that include 85 HBV positive samples from unidentified subjects from the Apollo Health City, Hyderabad, and 165 blood samples collected from ICRISAT campus, Hyderabad, India. All the samples that were positive to AFB1-lys were from HBV-positive subjects, indicating a potential risk of hepatocellular carcinoma in 4.8% of the subjects tested in the present study. This validation confirms the suitability of IC-ELISA, which is simple, cost-effective and enables high-throughput analysis.
dc.identifierhttps://hdl.handle.net/10568/83275
dc.identifier.urihttp://hdl.handle.net/123456789/89165
dc.languageen
dc.rightsLimited Access
dc.sourceAnitha, S., Waliyar, F., Reddy, A.S., Rao, R., Rao, R. & Kumar, P.L. (2011). Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans. Current Science, 101(7), 844-846.
dc.subjectaflatoxins
dc.subjecttoxin contamination
dc.subjectfungal infestation
dc.subjectaflatoxin exposure
dc.titleDevelopment of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans
dc.typeJournal Article

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Instituto Internacional de Investigación de Cultivos para las Zonas Tropicales Semiáridas (ICRISAT)