Technical note: A new and cost-effective method for detection of the bovine acyl-CoA: diacylglycerol acyltransferase 1 K232A polymorphism in cattle.

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dc.contributorR. S. STEINBERG, UFMG; L. PEREIRA, UFMG; G. A. LACORTE, UFMG; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, Pesquisador aposentado do CNPGL; MARCO ANTONIO MACHADO, CNPGL; C. G. FONSECA, UFMG; M. R. S. CARVALHO, UFMG.
dc.creatorSTEINBERG, R. S.
dc.creatorPEREIRA, L.
dc.creatorLACORTE, G. A.
dc.creatorPEIXOTO, M. G. C. D.
dc.creatorVERNEQUE, R. da S.
dc.creatorTEODORO, R. L.
dc.creatorMACHADO, M. A.
dc.creatorFONSECA, C. G.
dc.creatorCARVALHO, M. R. S.
dc.date2024-02-16T11:32:30Z
dc.date2024-02-16T11:32:30Z
dc.date2010-04-26
dc.date2009
dc.date.accessioned2026-07-07T03:51:21Z
dc.descriptionA new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferase1 (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT1 gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGAT1 K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGAT1 genotyping for gene-assisted selection.
dc.identifierJournal of Dairy Science, v. 92, n. 2, p. 773-776, 2009.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/711762
dc.identifier.urihttp://hdl.handle.net/123456789/444973
dc.languageeng
dc.rightsopenAccess
dc.subjectDGAT1
dc.titleTechnical note: A new and cost-effective method for detection of the bovine acyl-CoA: diacylglycerol acyltransferase 1 K232A polymorphism in cattle.
dc.typeArtigo de periódico

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