Gene expression and metabolite profiling of thirteen Nigerian cassava landraces to elucidate starch and carotenoid composition

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dc.creatorOlayide, P.
dc.creatorLarge, A.
dc.creatorStridh, L.
dc.creatorRabbi, Ismail Y.
dc.creatorBaldermann, S.
dc.creatorStavolone, L.
dc.creatorAlexandersson, E.
dc.date2020
dc.date2022-04-27T14:24:07Z
dc.date2022-04-27T14:24:07Z
dc.date.accessioned2026-06-27T15:58:37Z
dc.descriptionThe prevalence of vitamin A deficiency in sub-Saharan Africa necessitates effective approaches to improve provitamin A content of major staple crops. Cassava holds much promise for food security in sub-Saharan Africa, but a negative correlation between β-carotene, a provitamin A carotenoid, and dry matter content has been reported, which poses a challenge to cassava biofortification by conventional breeding. To identify suitable material for genetic transformation in tissue culture with the overall aim to increase β-carotene and maintain starch content as well as better understand carotenoid composition, root and leaf tissues from thirteen field-grown cassava landraces were analyzed for agronomic traits, carotenoid, chlorophyll, and starch content. The expression of five genes related to carotenoid biosynthesis were determined in selected landraces. Analysis revealed a weak negative correlation between starch and β-carotene content, whereas there was a strong positive correlation between root yield and many carotenoids including β-carotene. Carotenoid synthesis genes were expressed in both white and yellow cassava roots, but phytoene synthase 2 (PSY2), lycopene-ε-cyclase (LCYε), and β-carotenoid hydroxylase (CHYβ) expression were generally higher in yellow roots. This study identified lines with reasonably high content of starch and β-carotene that could be candidates for biofortification by further breeding or plant biotechnological means.
dc.formatapplication/pdf
dc.identifierhttps://hdl.handle.net/10568/119401
dc.identifier.urihttp://hdl.handle.net/123456789/119030
dc.languageen
dc.publisherMDPI
dc.rightsOpen Access
dc.sourceOlayide, P., Large, A., Stridh, L., Rabbi, I., Baldermann, S., Stavolone, L. & Alexandersson, E. (2020). Gene expression and metabolite profiling of thirteen Nigerian cassava landraces to elucidate starch and carotenoid composition. Agronomy, 10(3), 1-17.
dc.subjectcarotenoids
dc.subjectbiosynthesis
dc.subjectcassava
dc.subjectfood security
dc.subjectvitamin a
dc.subjectsubsaharan africa
dc.subjectnigeria
dc.titleGene expression and metabolite profiling of thirteen Nigerian cassava landraces to elucidate starch and carotenoid composition
dc.typeJournal Article

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Instituto Internacional de Agricultura Tropical (IITA)