MICROPROPAGATION AND ACCLIMATIZATION OF Aegiphila verticillata Vell.: AN ENDANGERED WOODY SPECIES

dc.creatorLuísa Maria Silveira de Almeida
dc.creatorLeandro Elias Morais
dc.creatorCristiano Ferrara de Resende
dc.creatorVirgínia Fernandes Braga
dc.creatorPaula da Fonseca Pereira
dc.creatorRodolpho Abrantes Camerini e Silva
dc.creatorPaulo Henrique Pereira Peixoto
dc.date2015
dc.date.accessioned2026-07-07T03:17:21Z
dc.descriptionThe objective of this work was to establish an efficient protocol for in vitro multiplication and rooting, as well as ex vitro acclimatization of Aegiphila verticillata, a woody species found in Brazilian rocky fields. Aseptic cultures were established by seeds and two multiplication analyses were performed. In the first, we employed 6-benzylaminopurine (BAP – 0, 2.5, 5 and 7.5 μM) + α-naphthalene acetic acid (NAA – 0, 0.2, 0.4 and 0.6 μM) and, in the second, were studied adenine sulfate, kinetin and thidiazuron (0, 5, 7.5, 10 and 12.5 μM). After 90 days, we assessed the quantitative and qualitative shoot propagation. There were more than 90% seed germination and low contamination (2%). In multiplication phase, the culture medium that promoted the best quantitative and qualitative culture development was supplemented with 7.5 μM BAP + 0.4 μM NAA. In the rooting assay, were used NAA, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) (0, 0.1, 0.2, 0.3 or 0.4 μM). After 90 days, the root number and rooting quality were evaluated. In this analysis, differences were not found between the control and the other treatments. Rooted plantlets were acclimatized in styrofoam trays for 30 days, after which they were transferred to pots in the greenhouse. Only 3% of the plants subjected to initial acclimatization died and 70% of the plants transferred to the field conditions survived and showed normal development. The results founded in this work are the first involving in vitro propagation and ex vitro acclimatization of Aegiphila verticillata and provide a continuous supply of this medicinal native species, endangered due anthropogenic activities.
dc.formatapplication/pdf
dc.identifier0100-6762
dc.identifierhttps://www.redalyc.org/articulo.oa?id=48839479010
dc.identifier.urihttp://hdl.handle.net/123456789/429244
dc.languageen
dc.publisherUniversidade Federal de Viçosa
dc.relationhttp://www.redalyc.org/revista.oa?id=488
dc.rightsRevista Árvore
dc.sourceRevista Árvore (Brasil) Num.2 Vol.39
dc.subjectAgrociencias
dc.titleMICROPROPAGATION AND ACCLIMATIZATION OF Aegiphila verticillata Vell.: AN ENDANGERED WOODY SPECIES
dc.typeartículo científico

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