Monitoring stimulated darkening from UV-C light on different bean genotypes by NMR spectroscopy.

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dc.contributorLUIZ ALBERTO COLNAGO, CNPDIA.
dc.creatorSALVADOR, M. V.
dc.creatorKOCK, F. V. C.
dc.creatorSANTOS, I. L.
dc.creatorPAULINO, J. F. C.
dc.creatorALMEIDA, C. P. de
dc.creatorGARCIA, R. H. S.
dc.creatorBENCHIMOL-REIS, L. L.
dc.creatorCOLNAGO, L. A.
dc.creatorFERREIRA, A. G.
dc.date2022-04-28T05:03:19Z
dc.date2022-04-28T05:03:19Z
dc.date2022-04-27
dc.date2022
dc.date.accessioned2026-07-07T04:28:51Z
dc.descriptionThe use of UV-C cool white light on bean (Phaseolus vulgaris L.) seeds significantly increases the biochemical seed coat post-harvest darkening process, whilst preserving seed germination. The aim of this work consists in monitoring the effect caused by the incidence of UV-C light on different bean genotypes using NMR spectroscopy. The genotype samples named IAC Alvorada; TAA Dama;BRS Estilo and BRS Pérola from the Agronomic Institute (IAC; Campinas; SP; Brazil) were evaluated.The following two methodologies were used: a prolonged darkening, in which the grain is placed in a room at a controlled temperature (298 K) and humidity for 90 days, simulating the supermarket shelf; an accelerated darkening, where the grains are exposed to UV-C light (254 nm) for 96 h. The experiments were performed using the following innovative time-domain (TD) NMR approaches:the RK-ROSE pulse sequence; one- and two-dimensional high resolution (HR) NMR experiments(1H; 1H-1H COSY and 1H-13C HSQC); chemometrics tools, such as PLS-DA and heat plots. The results suggest that the observed darkening occurs on the tegument after prolonged (90 days) and accelerated (96 h) conditions. In addition, the results indicate that phenylalanine is the relevant metabolite within this context, being able to participate in the chemical reactions accounted for by the darkening processes. Additionally, it is possible to confirm that a UV-C lamp accelerates oxidative enzymatic reactions and that the NMR methods used were a trustworthy approach to monitor and understand the darkening in bean seeds at metabolite level.
dc.format10 p.
dc.identifierMolecules, v. 27, 2060, 2022.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1142447
dc.identifierhttps://doi.org/10.3390/molecules27072060
dc.identifier.urihttp://hdl.handle.net/123456789/462834
dc.languageeng
dc.rightsopenAccess
dc.subjectBean darkening
dc.subjectUV-C irradiation
dc.subjectNMR spectroscopy and chemometrics
dc.titleMonitoring stimulated darkening from UV-C light on different bean genotypes by NMR spectroscopy.
dc.typeArtigo de periódico

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