Anatomical and biochemical responses to oxidative stress in shoots of Bambusa vulgaris Schrad. ex Wendl during the in vitro-ex vitro transition
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Hyperhydricity can affect the development of plant morphology. A better understanding of the anatomical
and physiological changes of hyperhydric plants is needed to predict and control the occurrence of
hyperhydryc conditions. The aim of this study is to demonstrate the role of oxidative stress in
hyperhydricity. To this end, the anatomical, physiological and biochemical responses to oxidative stress
in shoots of Bambusa vulgaris Schrad. ex Wendl were compared during the in vitro-ex vitro transition. For
this propose, we used shoots grown in two different culture systems: liquid static culture medium and
temporary immersion system. Our results showed that hyperhydricity was associated with oxidative
stress in the shoots. In hyperhydric shoots chlorophyll content decreased when cultured in liquid static
culture medium. Moreover, hydrogen peroxide content and malondialdehyde, as well as the activities of
catalase and enzymes of ascorbate-glutathione cycle (ascorbate peroxidase, monodehydroascorbate
reductase and dehydroascorbate reductase) were increased in these shoots. On the other hand, scanning
electron microscopy showed that the leaves of hyperhydric shoots exhibited anatomical changes in the
stomata of the plants, whereas the leaves of normal shoots showed normal structural development.
Finally, normal shoots showed high survival rate and allowed better adaptation of the plantlets in the
greenhouse.
Educational Network in Agrobiodiversity - EDUNABIO
Educational Network in Agrobiodiversity - EDUNABIO
Palabras clave
Genética vegetal y fitomejoramiento - F30, Bambusa vulgaris, Morfología, Brote, In vitro, Transversal, http://aims.fao.org/aos/agrovoc/c_805, http://aims.fao.org/aos/agrovoc/c_49903, http://aims.fao.org/aos/agrovoc/c_14261, http://aims.fao.org/aos/agrovoc/c_37563