In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches

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dc.creatorLorieux, Mathias
dc.creatorBlein, Mélisande
dc.creatorLozano, Jaime
dc.creatorBouniol, Matthieu
dc.creatorDroc, Gaëtan
dc.creatorDiévart, Anne
dc.creatorPérin, Christophe
dc.creatorMieulet, Delphine
dc.creatorLanau, Nadege
dc.creatorBès, Martine
dc.creatorRouvière, Claire
dc.creatorGay, Céline
dc.creatorPiffanelli, Pietro
dc.creatorLarmande, Pierre
dc.creatorMichel, Corinne
dc.creatorBarnola, Isabelle
dc.creatorBiderre-Petit, Corinne
dc.creatorSallaud, Christophe
dc.creatorPérez, Pascual
dc.creatorBourgis, Fabienne
dc.creatorGhesquière, Alain
dc.creatorGantet, Pascual
dc.creatorTohme, Joseph M.
dc.creatorMorel, Jean-Benoit
dc.creatorGuiderdoni, Emmanuel
dc.date2012-06
dc.date2017-10-12T08:02:05Z
dc.date2017-10-12T08:02:05Z
dc.date.accessioned2026-06-27T14:35:14Z
dc.descriptionWe report here the molecular and phenotypic features of a library of 31 562 insertion lines generated in the model japonica cultivar Nipponbare of rice (Oryza sativa L.), called Oryza Tag Line (OTL). Sixteen thousand eight hundred and fourteen T‐DNA and 12 410 Tos17 discrete insertion sites have been characterized in these lines. We estimate that 8686 predicted gene intervals—i.e. one‐fourth to one‐fifth of the estimated rice nontransposable element gene complement—are interrupted by sequence‐indexed T‐DNA (6563 genes) and/or Tos17 (2755 genes) inserts. Six hundred and forty‐three genes are interrupted by both T‐DNA and Tos17 inserts. High quality of the sequence indexation of the T2 seed samples was ascertained by several approaches. Field evaluation under agronomic conditions of 27 832 OTL has revealed that 18.2% exhibit at least one morphophysiological alteration in the T1 progeny plants. Screening 10 000 lines for altered response to inoculation by the fungal pathogen Magnaporthe oryzae allowed to observe 71 lines (0.7%) developing spontaneous lesions simulating disease mutants and 43 lines (0.4%) exhibiting an enhanced disease resistance or susceptibility. We show here that at least 3.5% (four of 114) of these alterations are tagged by the mutagens. The presence of allelic series of sequence‐indexed mutations in a gene among OTL that exhibit a convergent phenotype clearly increases the chance of establishing a linkage between alterations and inserts. This convergence approach is illustrated by the identification of the rice ortholog of AtPHO2, the disruption of which causes a lesion‐mimic phenotype owing to an over‐accumulation of phosphate, in nine lines bearing allelic insertions.
dc.identifierhttps://hdl.handle.net/10568/88270
dc.identifier.urihttp://hdl.handle.net/123456789/82468
dc.languageen
dc.publisherWiley
dc.rightsLimited Access
dc.sourceLorieux, Mathias; Blein, Mélisande; Lozano, Jaime; Bouniol, Matthieu; Droc, Gaëtan; Diévart, Anne; Périn, Christophe; Mieulet, Delphine; Lanau, Nadege; Bès, Martine; Rouvière, Claire; Gay, Céline; Piffanelli, Pietro; Larmande, Pierre; Michel, Corinne; Barnola, Isabelle; Biderre-Petit, Corinne; Sallaud, Christophe; Perez, Pascual; Bourgis, Fabienne; Ghesquière, Alain; Gantet, Pascual; Tohme, Joseph M.; Morel, Jean Benoit; Guiderdoni, Emmanuel. 2012. In-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches. Plant Biotechnology Journal. 10(5) 555-568.
dc.subjectoryza sativa
dc.subjectrice
dc.subjectmutagenesis
dc.subjectmolecular genetics
dc.subjectphenotypes
dc.subjectdna
dc.subjectarroz
dc.subjectmutagénesis
dc.subjectgenética molecular
dc.subjectfenotipos
dc.subjectadn
dc.titleIn-depth molecular and phenotypic characterization in a rice insertion line library facilitates gene identification through reverse and forward genetics approaches
dc.typeJournal Article

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Centro Internacional de Agricultura Tropical (CIAT)