Study on single nucleotide polymorphisms of H -FABP gene in sheep

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dc.creatorQiao, H.Y.
dc.creatorZhao, Q.J.
dc.creatorYao, N.
dc.creatorLu, Y.-Z.
dc.creatorMeng, X.-R.
dc.creatorGuan, Wei-Jun
dc.creatorMa, Y.H.
dc.creatorHan Jianlin
dc.date2009-08-14
dc.date2010-08-04T13:29:08Z
dc.date2010-08-04T13:29:08Z
dc.date.accessioned2026-06-27T16:36:13Z
dc.descriptionPCR-SSCP and DNA sequencing approaches were applied to assess the single nucleotide polymorphisms (SNPs) and analyze the genetic polymorphisms at partial exon 2 and intron 2 of H-FABP(Heart fatty acid-binding protein)gene, and five sheep populations that comprised of Small-Tailed Han sheep (SH, 48), Ningxia Tan sheep (Tan, 121), Tan × SH F1 (23), Poll Dorset (48) and Suffolk (24) sheep were screened in this stuty. The result showed: (1)four SNPs at 981(G/A), 1014(A/C) 1019(T/C) and 1058 (-/G) and nine genotypes (AA, BB, CC, AB, AC, BC, AD, CD and BD) were detected using primer 2, the AA was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium except the Tan and Suffolk populations. Statistical analysis revealed a low polymorphism information content (PIC) in the Suffolk and Tan × SH F1 populations (PIC< 0.25) but an intermediate PIC in the remaining three populations (0.25< PIC <0.50). It meant that the fragment of H-FABP had polymorphisms, which could be used as a candidate gene associated gene with phenotypic traits like intramuscular fat content in different sheep populations. (2)Three genotypes (HH, Hh and hh) determined by a SNP at 2407(T-C) were detected using primer 4. The genotype frequencies were in the order of HH＞Hh＞hh. A chi-square analy-sis suggested that the allele frequencies and genotype frequencies of H-FABP were in Hardy-Weinberg equilibrium in the Tan and Poll Dorset populations, and the PIC values were low (PIC < 0.25). However, there was no polymorphisms in SH, Tan × SH F1 and Suffolk populations.
dc.identifierhttps://hdl.handle.net/10568/2168
dc.identifier.urihttp://hdl.handle.net/123456789/127855
dc.languagezh
dc.publisherChina Science Publishing & Media Ltd.
dc.rightsLimited Access
dc.sourceQiao, H.-Y., Zhao, Q.-J., Yao, N., Lu, Y.-Z., Meng, X.-R., Jianlin, H., Guan, W.-J.; Ma, Y.-H. 2009. Study on single nucleotide polymorphisms of H -FABP gene in sheep. Hereditas (Beijing) 31(7):725-731.
dc.subjectgenetics
dc.subjectsheep
dc.titleStudy on single nucleotide polymorphisms of H -FABP gene in sheep
dc.typeJournal Article

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