Plantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species.

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dc.contributorCarolina Mariane MOREIRA, UFLA; Helena Botelho de ANDRADE, UFLA; Suzan Kelly Vilela BERTOLUCCI, UFLA; OSMAR ALVES LAMEIRA, CPATU; Aliyu MOHAMMED, Abant ?zzet Baysal University; José Eduardo Brasil Pereira PINTO, UFLA.
dc.creatorMOREIRA, C. M.
dc.creatorANDRADE, H. B. de
dc.creatorBERTOLUCCI, S. K. V.
dc.creatorLAMEIRA, O. A.
dc.creatorMOHAMMED, A.
dc.creatorPINTO, J. E. B. P.
dc.date2017-01-02T11:11:11Z
dc.date2017-01-02T11:11:11Z
dc.date2017-01-02
dc.date2016
dc.date2017-09-06T11:11:11Z
dc.date.accessioned2026-06-30T23:43:50Z
dc.descriptionThe present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6- trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%.
dc.identifierTurkish Journal of Biology, v. 40, n. 6, p. 1227-1234, 2016.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1059742
dc.identifier10.3906/biy-1601-56
dc.identifier.urihttp://hdl.handle.net/123456789/388983
dc.languageeng
dc.rightsopenAccess
dc.subjectRegeneração in vitro
dc.subjectCuraua
dc.subjectRegeneração
dc.subjectthidiazuron
dc.titlePlantlet regeneration from young leaf segments of curaua (Ananas erectifolius), an Amazon species.
dc.typeArtigo de periódico

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Embrapa Amazonía Oriental (CPATU)