Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia

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dc.creatorHaile, A.F.
dc.creatorAlonso, Silvia
dc.creatorBelay, N.
dc.creatorAtoma, T.B.
dc.creatorBoyaka, P.N.
dc.creatorGrace, Delia
dc.date2022-02-24
dc.date2022-02-24T10:46:12Z
dc.date2022-02-24T10:46:12Z
dc.date.accessioned2026-06-27T17:13:39Z
dc.descriptionEscherichia coli O157:H7 is an emerging foodborne pathogen of public health importance. The objectives of this study were to estimate the prevalence and evaluate the antimicrobial susceptibility pattern and multidrug-resistant profile of E. coli O157:H7 isolated from raw beef sold in butcher shops in Addis Ababa, Ethiopia. A total of 384 raw beef samples were collected from randomly selected butcher shops across the 10 sub-cities of Addis Ababa. E. coli O157:H7 was isolated following ISO-16654:2001 standard, and isolates were tested for resistance to 13 antimicrobial agents using the Kirby–Bauer disk diffusion method. Out of the 384 retail raw beef samples examined, 14 (3.64%) (95% CI = 1.77–5.51%) carried E. coli O157:H7 serotype. Of the 14 E. coli O157:H7 isolates, 8 (57.14%) were found to be resistant to three or more antimicrobial categories. The frequency of resistant phenotype was more common for ampicillin (92.8%), nitrofurantoin (92.8%), and tetracycline (50%). Multidrug-resistant E. coli O157:H7 were present in raw beef sold in butcher shops in Addis Ababa. Thus, more stringent monitoring of antimicrobial use in both human and animal populations should be implemented. In addition, further studies should be conducted to understand the E. coli O157:H7 points of contamination and define appropriate risk mitigation strategies.
dc.identifierhttps://hdl.handle.net/10568/118241
dc.identifier.urihttp://hdl.handle.net/123456789/146005
dc.languageen
dc.publisherFrontiers Media
dc.rightsOpen Access
dc.sourceHaile, A.F., Alonso, S., Belay, N., Atoma, T.B., Boyaka, P.N. and Grace, D. 2022. Prevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia. Frontiers in Veterinary Science 9: 734896.
dc.subjectantimicrobial resistance
dc.subjectfood safety
dc.subjectanimal products
dc.subjectmeat
dc.titlePrevalence, antibiogram, and multidrug-resistant profile of E. coli O157: H7 in retail raw beef in Addis Ababa, Ethiopia
dc.typeJournal Article

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