Analysis of the cellular immune responses to vaccines

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dc.creatorSvitek, Nicholas
dc.creatorTaracha, E.L.N.
dc.creatorSaya, R.
dc.creatorAwino, Elias
dc.creatorNene, Vishvanath M.
dc.creatorSteinaa, Lucilla
dc.date2016
dc.date2016-03-20T07:36:00Z
dc.date2016-03-20T07:36:00Z
dc.date.accessioned2026-06-27T16:59:29Z
dc.descriptionFlow cytometry, enzyme-linked immunospot (ELISpot) and cellular cytotoxicity assays are powerful tools for studying the cellular immune response towards intracellular pathogens and vaccines in livestock species. Lymphocytes from immunized animals can be purified using Ficoll-Paque density gradient centrifugation and evaluated for their antigen specificity or reactivity towards a vaccine. Here, we describe staining of bovine lymphocytes with peptide (p)-MHC class I tetramers and antibodies specific towards cellular activation markers for evaluation by multiparametric flow cytometry, as well as interferon (IFN)-γ ELISpot and cytotoxicity using chromium (51Cr) release assays. A small component on the use of immunoinformatics for fine-tuning the identification of a minimal CTL epitope is included.
dc.identifierhttps://hdl.handle.net/10568/72681
dc.identifier.urihttp://hdl.handle.net/123456789/139013
dc.languageen
dc.publisherSpringer
dc.rightsLimited Access
dc.sourceSvitek, N., Taracha, E.L., Saya, R., Awino, E., Nene, V. and Steinaa, L. 2016. Analysis of the cellular immune responses to vaccines. IN: Brun, A. (ed), Vaccine technologies for veterinary viral diseases: Methods and protocols, vol. 1349. Dordrecht: Springer. pp. 247-262.
dc.subjectvaccines
dc.titleAnalysis of the cellular immune responses to vaccines
dc.typeBook Chapter

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Instituto Internacional de Investigaciones Pecuarias (ILRI)